Ubiquitin-conjugating enzyme E2 N - Revision history

Wu3857: /* Reference */

2019-08-19T23:02:03Z

‎Reference

Wu3857: /* Protein Function */

2019-08-19T02:51:43Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-19T02:27:56Z

‎Protein Function

Wu3857 at 20:40, 7 August 2019

2019-08-07T20:40:00Z

Wu3857 at 01:26, 4 August 2019

2019-08-04T01:26:53Z

Wu3857 at 01:25, 4 August 2019

2019-08-04T01:25:57Z

Wu3857: /* Reference */

2019-08-04T01:22:39Z

‎Reference

Wu3857: /* Structural Information */

2019-08-04T01:21:15Z

‎Structural Information

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/..."

2019-08-04T01:20:19Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/..."

New page

{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/family/P61088 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 N|Ligand list]]|Ubiquitinase/Deubiquitinase}}
==Summary==

===Protein Function ===
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. (From Uniprot) 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. 

===Cys Function & Property===
Cys87 is the active site of UBE2N, which could form a glycyl thioester intermediate during the catalysis. 

* Hydrophobic property:
:[[File:524-hydro.png||600px]]
* SASA：
:Cys87: 27.384 A^2

==Protein Sequence==

MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG 
TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRI'''C'''LDI LKDKWSPALQ 
IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN 
NI 


==Structural Information==
*Known structures with covalent ligands: 
:[https://www.rcsb.org/structure/4ONN 4ONN] 

*Protein structure:
[[File:524.PNG|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04120 Ubiquitin mediated proteolysis] 

==Experimental Evidence==
:MALDI-TOF/MS, Tryptic Digest, Crystallography

==Reference==
# Strickson S, Campbell D G, Emmerich C H, et al. '''The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system[J].''' Biochemical Journal, 2013, 451(3): 427-437. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23441730 23441730] 
# Hodge C D, Edwards R A, Markin C J, et al. '''Covalent inhibition of Ubc13 affects ubiquitin signaling and reveals active site elements important for targeting[J].''' ACS chemical biology, 2015, 10(7): 1718-1728. [https://www.ncbi.nlm.nih.gov/pubmed/?term=25909880 25909880] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]

@@ Line 44: / Line 44: @@
 # Hodge C D, Edwards R A, Markin C J, et al. '''Covalent inhibition of Ubc13 affects ubiquitin signaling and reveals active site elements important for targeting[J].''' ACS chemical biology, 2015, 10(7): 1718-1728. [https://www.ncbi.nlm.nih.gov/pubmed/?term=25909880 25909880]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
+[[Category:Ubiquitinase/Deubiquitinase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
+[[Category:Ubiquitin-conjugating enzyme family]]
-[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
+[[Category:Ubiquitin mediated proteolysis]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. (From Uniprot)<br/>
-S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. <br/>
+S-ubiquitinyl-[E1]-L-cysteine + [E2]-L-cysteine = [E1]-L-cysteine + S-ubiquitinyl-[E2]-L-cysteine. <br/>
 [[File:571-function-UB.jpg|center|1000px]]
 <div align="center">PMID: 27002218</div>

@@ Line 8: / Line 8: @@
 The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. (From Uniprot)<br/>
 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. <br/>
 ===Cys Function & Property===

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 N|Ligand list]]|Ubiquitinase/Deubiquitinase}}
+{{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys87|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 N|Ligand list]]|Ubiquitinase/Deubiquitinase}}
 ==Summary==

@@ Line 30: / Line 30: @@
 *Protein structure:
-[[File:524.PNG|center|800px]]
+[[File:524.png|center|800px]]
 ==Related Pathway==

Ubiquitin-conjugating enzyme E2 N - Revision history

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857 at 20:40, 7 August 2019

Wu3857 at 01:26, 4 August 2019

Wu3857 at 01:25, 4 August 2019

Wu3857: /* Reference */

Wu3857: /* Structural Information */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Ubc13, UbcH13, UBE2N|[https://www.uniprot.org/uniprot/P61088 P61088]|Homo sapiens|Cys674|[http://pfam.xfam.org/..."