Ubiquitin-conjugating enzyme E2 L3 - Revision history

Wu3857: /* Reference */

2019-08-19T23:02:43Z

‎Reference

Wu3857: /* Protein Function */

2019-08-19T02:46:26Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-19T02:45:58Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-19T02:45:22Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-19T02:45:09Z

‎Protein Function

Wu3857: /* Summary */

2019-08-19T02:44:46Z

‎Summary

Wu3857: /* Protein Function */

2019-08-19T02:27:36Z

‎Protein Function

Wu3857 at 20:39, 7 August 2019

2019-08-07T20:39:35Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[..."

2019-08-04T01:29:23Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[..."

New page

{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 L3|Ligand list]]|Ubiquitinase/Deubiquitinase}}
==Summary==

===Protein Function ===
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. (From Uniprot) 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. 

===Cys Function & Property===
Cys87 is the active site of UBE2N, which could form a glycyl thioester intermediate during the catalysis. 

* Hydrophobic property:
:[[File:525-hydro.png||600px]]
* SASA：
:Cys87: 32.715 A^2

==Protein Sequence==

MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA 
FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQV'''C'''LPVI SAENWKPATK 
TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK 
RPVD 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:525.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04120 Ubiquitin mediated proteolysis] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05012 Parkinson disease] 

==Experimental Evidence==
:MALDI-TOF/MS, Tryptic Digest

==Reference==
# Strickson S, Campbell D G, Emmerich C H, et al. '''The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system[J].''' Biochemical Journal, 2013, 451(3): 427-437. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23441730 23441730] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
[[Category:Parkinson disease|Parkinson disease]]

@@ Line 46: / Line 46: @@
 # Strickson S, Campbell D G, Emmerich C H, et al. '''The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system[J].''' Biochemical Journal, 2013, 451(3): 427-437. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23441730 23441730]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
+[[Category:Ubiquitinase/Deubiquitinase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
+[[Category:Ubiquitin-conjugating enzyme family]]
-[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
+[[Category:Ubiquitin mediated proteolysis]]
-[[Category:Parkinson disease|Parkinson disease]]
+[[Category:Parkinson disease]]

@@ Line 9: / Line 9: @@
 UBE2L3 expressed in 226 organ(s), highest expression level in C1 segment of cervical spinal cord. <br/>
 The reaction of UBE2L3: <br/>
-<div align="center">S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</div>
+<div align="center">S-ubiquitinyl-[E1]-L-cysteine + [E2]-L-cysteine = [E1]-L-cysteine + S-ubiquitinyl-[E2]-L-cysteine.</div>
 [[File:571-function-UB.jpg|center|1000px]]
 <div align="center">PMID: 27002218</div>

@@ Line 9: / Line 9: @@
 UBE2L3 expressed in 226 organ(s), highest expression level in C1 segment of cervical spinal cord. <br/>
 The reaction of UBE2L3: <br/>
-S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. <br/>
+<div align="center">S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</div>
 [[File:571-function-UB.jpg|center|1000px]]
 <div align="center">PMID: 27002218</div>

@@ Line 8: / Line 8: @@
 Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. (From Uniprot)<br/>
 UBE2L3 expressed in 226 organ(s), highest expression level in C1 segment of cervical spinal cord. <br/>
-The reaction of UBE2L3:
+The reaction of UBE2L3: <br/>
 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. <br/>
 [[File:571-function-UB.jpg|center|1000px]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. (From Uniprot)<br/>
-Expressed in 226 organ(s), highest expression level in C1 segment of cervical spinal cord. <br/>
+UBE2L3 expressed in 226 organ(s), highest expression level in C1 segment of cervical spinal cord. <br/>
 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine. <br/>
 [[File:571-function-UB.jpg|center|1000px]]

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 L3|Ligand list]]|Ubiquitinase/Deubiquitinase}}
+{{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 L3|Ligand list]]|Ubiquitinase/Deubiquitinase,<br/>Post-translational Modification}}
 ==Summary==
@@ Line 45: / Line 45: @@
 [[Category:Homo sapiens|Homo sapiens]]
 [[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
 [[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
 [[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
 [[Category:Parkinson disease|Parkinson disease]]

Ubiquitin-conjugating enzyme E2 L3 - Revision history

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: /* Summary */

Wu3857: /* Protein Function */

Wu3857 at 20:39, 7 August 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|UbcH7, UBE2L3, Ubiquitin-protein ligase L3|[https://www.uniprot.org/uniprot/P68036 P68036]|Homo sapiens|Cys86|[..."