Wu3857: /* Reference */

2019-08-19T23:04:03Z

‎Reference

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|UBE2D2|[https://www.uniprot.org/uniprot/P62837 P62837]|Homo sapiens|Cys85|[http://pfam.xfam.org/family/PF00179..."

2019-08-19T02:49:56Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|UBE2D2|[https://www.uniprot.org/uniprot/P62837 P62837]|Homo sapiens|Cys85|[http://pfam.xfam.org/family/PF00179..."

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{{#invoke:InfoboxforTarget|run|UBE2D2|[https://www.uniprot.org/uniprot/P62837 P62837]|Homo sapiens|Cys85|[http://pfam.xfam.org/family/PF00179 Ubiquitin-conjugating enzyme family]|[[:Category:Ubiquitin-conjugating enzyme E2 D2|Ligand list]]|Post-translational Modification, Ubiquitinase/Deubiquitinase}}
==Summary==

===Protein Function ===
Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell. 
UBE2D2 expressed in 236 organs, highest expression level in testis.
[[File:571-function-UB.jpg|center|1000px]]
<div align="center">PMID: 27002218</div>

===Cys Function & Property===
Cys85 is the active site of UBE2D2, act as a glycyl thioester intermediate. 

* Hydrophobic property:
:[[File:573-hydro.png||600px]]
* SASA:
:Cys85: 18.529 A^2 

==Protein Sequence==

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF 
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSI'''C'''LDILR SQWSPALTIS 
KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:[[File:573.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04120 Ubiquitin mediated proteolysis] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04141 Protein processing in endoplasmic reticulum] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05131 Shigellosis] 

==Experimental Evidence==
:LC-CID-MS/MS, Tryptic Digest

==Reference==
# Liu L, Hua Y, Wang D, et al. '''A sesquiterpene lactone from a medicinal herb inhibits proinflammatory activity of TNF-α by inhibiting ubiquitin-conjugating enzyme UbcH5[J].''' Chemistry & biology, 2014, 21(10): 1341-1350. [https://www.ncbi.nlm.nih.gov/pubmed/?term=25200604 25200604] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
[[Category:Post-translational Modification|Post-translational Modification]]
[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
[[Category:Protein processing in endoplasmic reticulum|Protein processing in endoplasmic reticulum]]
[[Category:Shigellosis|Shigellosis]]

@@ Line 44: / Line 44: @@
 # Liu L, Hua Y, Wang D, et al. '''A sesquiterpene lactone from a medicinal herb inhibits proinflammatory activity of TNF-α by inhibiting ubiquitin-conjugating enzyme UbcH5[J].''' Chemistry & biology, 2014, 21(10): 1341-1350. [https://www.ncbi.nlm.nih.gov/pubmed/?term=25200604 25200604]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Ubiquitinase/Deubiquitinase|Ubiquitinase/Deubiquitinase]]
+[[Category:Ubiquitinase/Deubiquitinase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:Ubiquitin-conjugating enzyme family|Ubiquitin-conjugating enzyme family]]
+[[Category:Ubiquitin-conjugating enzyme family]]
-[[Category:Ubiquitin mediated proteolysis|Ubiquitin mediated proteolysis]]
+[[Category:Ubiquitin mediated proteolysis]]
-[[Category:Protein processing in endoplasmic reticulum|Protein processing in endoplasmic reticulum]]
+[[Category:Protein processing in endoplasmic reticulum]]
-[[Category:Shigellosis|Shigellosis]]
+[[Category:Shigellosis]]

Ubiquitin-conjugating enzyme E2 D2 - Revision history

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|UBE2D2|[https://www.uniprot.org/uniprot/P62837 P62837]|Homo sapiens|Cys85|[http://pfam.xfam.org/family/PF00179..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|UBE2D2|[https://www.uniprot.org/uniprot/P62837 P62837]|Homo sapiens|Cys85|[http://pfam.xfam.org/family/PF00179..."