Wu3857: /* Reference */

2019-08-19T23:19:25Z

‎Reference

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|TUBB2A, TUBB2|[https://www.uniprot.org/uniprot/Q13885 Q13885]|Homo sapiens|Cys239|[http://pfam.xfam.org/family/..."

2019-08-16T02:58:21Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|TUBB2A, TUBB2|[https://www.uniprot.org/uniprot/Q13885 Q13885]|Homo sapiens|Cys239|[http://pfam.xfam.org/family/..."

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{{#invoke:InfoboxforTarget|run|TUBB2A, TUBB2|[https://www.uniprot.org/uniprot/Q13885 Q13885]|Homo sapiens|Cys239|[http://pfam.xfam.org/family/PF00091 Tubulin family]|[[:Category:Tubulin beta-2A chain|Ligand list]]|Structural protein}}

==Summary==

===Protein Function ===
Tubulin beta-2A chain is a protein that in humans is encoded by the TUBB2A gene. 
Tubulin (tubul- + -in) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. The tubulin superfamily contains six families of tubulins (alpha-, beta-, gamma-, delta-, epsilon and zeta-tubulins). Tubulin is also used to specifically refer to α-tubulin and β-tubulin, the proteins that make up microtubules in eukaryotic cells. Each has a molecular weight of approximately 50,000 Daltons. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be related to tubulin. 
Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. The β-tubulin subunit is exposed on the plus end of the microtubule while the α-tubulin subunit is exposed on the minus end. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the β-tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Whether the β-tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule. (From Wikipedia) 
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. (From Uniprot) 

===Cys Function & Property===
Cys-239 represents the common site of covalent modification on all susceptible b-tubulin isotypes. (PMID: 10318945) 

* Hydrophobic property:
:[[File:561-hydro.png||600px]]
* SASA：
:Cys274: :Unknown 

==Protein Sequence==

MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGSYHGDS DLQLERINVY 
YNEAAGNKYV PRAILVDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN 
WAKGHYTEGA ELVDSVLDVV RKESESCDCL QGFQLTHSLG GGTGSGMGTL 
LISKIREEYP DRIMNTFSVM PSPKVSDTVV EPYNATLSVH QLVENTDETY 
SIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTT'''C'''L RFPGQLNADL 
RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQMFDSKNMM 
AACDPRHGRY LTVAAIFRGR MSMKEVDEQM LNVQNKNSSY FVEWIPNNVK 
TAVCDIPPRG LKMSATFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG 
EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEQGEFEEEE GEDEA 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown 

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04145 Phagosome] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04540 Gap junction] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05130 Pathogenic Escherichia coli infection] 

==Experimental Evidence==
:Isotope Labeling, Edman Degradation

==Reference==
# Shan B, Medina J C, Santha E, et al. '''Selective, covalent modification of β-tubulin residue Cys-239 by T138067, an antitumor agent with in vivo efficacy against multidrug-resistant tumors[J].''' Proceedings of the National Academy of Sciences, 1999, 96(10): 5686-5691. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10318945 10318945] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Structural protein|Structural protein]]
[[Category:Tubulin family|Tubulin family]]
[[Category:Phagosome|Phagosome]]
[[Category:Gap junction|Gap junction]]
[[Category:Pathogenic Escherichia coli infection|Pathogenic Escherichia coli infection]]

@@ Line 51: / Line 51: @@
 # Shan B, Medina J C, Santha E, et al. '''Selective, covalent modification of β-tubulin residue Cys-239 by T138067, an antitumor agent with in vivo efficacy against multidrug-resistant tumors[J].''' Proceedings of the National Academy of Sciences, 1999, 96(10): 5686-5691. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10318945 10318945]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Structural protein|Structural protein]]
+[[Category:Structural protein]]
-[[Category:Tubulin family|Tubulin family]]
+[[Category:Tubulin family]]
-[[Category:Phagosome|Phagosome]]
+[[Category:Phagosome]]
-[[Category:Gap junction|Gap junction]]
+[[Category:Gap junction]]
-[[Category:Pathogenic Escherichia coli infection|Pathogenic Escherichia coli infection]]
+[[Category:Pathogenic Escherichia coli infection]]

Tubulin beta-2A chain - Revision history

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|TUBB2A, TUBB2|[https://www.uniprot.org/uniprot/Q13885 Q13885]|Homo sapiens|Cys239|[http://pfam.xfam.org/family/..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|TUBB2A, TUBB2|[https://www.uniprot.org/uniprot/Q13885 Q13885]|Homo sapiens|Cys239|[http://pfam.xfam.org/family/..."