Thioredoxin reductase TR1 (Rattus norvegicus) - Revision history

Wu3857: /* Reference */

2019-08-19T23:16:58Z

‎Reference

Wu3857: /* Cys Function & Property */

2019-08-04T02:25:09Z

‎Cys Function & Property

Wu3857 at 02:22, 4 August 2019

2019-08-04T02:22:06Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/fa..."

2019-08-04T02:21:38Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/fa..."

New page

{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/family/PF02852 Pyridine nucleotide-disulphide oxidoreductase] Class-I pyridine nucleotide-disulfide oxidoreductase family|[[:Category:Thioredoxin reductase TR1 (Rattus norvegicus)|Ligand list]]|Redox protein}}

==Summary==

===Protein Function ===
Thioredoxin reductases (TR, TrxR) are the only known enzymes to reduce thioredoxin (Trx). Two classes of thioredoxin reductase have been identified: one class in bacteria and some eukaryotes and one in animals. Both classes are flavoproteins which function as homodimers. Each monomer contains a FAD prosthetic group, a NADPH binding domain, and an active site containing a redox-active disulfide bond. 
Mammalian thioredoxin reductase mechanism: Mammalian TrxRs have a much higher sequence homology with glutathione reductase than E. coli. The active-site Cys residues in the FAD domain and bound NADPH domain are in close proximity removing the necessity for a 66 degree rotation for electron transfer found in E. coli. An additional feature of the mammalian mechanism is the presence of a selenocystine residue at the C-terminal end of the protein which is required for catalytic activity. The conserved residues in mammalian active site are -Cys-Val-Asn-Val-Gly-Cys-. (From Wikipedia) 

===Cys Function & Property===
Cys497 is very close to the active site of His472 in space. 

* Hydrophobic property:
:[[File:528-hydro.png||600px]]
* SASA：
:Cys497: 30.416 A^2

==Protein Sequence==

MNDSKDAPKS YDFDLIIIGG GSGGLAAAKE AAKFDKKVMV LDFVTPTPLG 
TRWGLGGTCV NVGCIPKKLM HQAALLGQAL KDSRNYGWKL EDTVKHDWEK 
MTESVQNHIG SLNWGYRVAL REKKVVYENA YGKFIGPHKI MATNNKGKEK 
VYSAERFLIA TGERPRYLGI PGDKEYCISS DDLFSLPYCP GKTLVVGASY 
VALECAGFLA GIGLDVTVMV RSILLRGFDQ DMANKIGEHM EEHGIKFIRQ 
FVPTKIEQIE AGTPGRLKVT AKSTNSEETI EDEFNTVLLA VGRDSCTRTI 
GLETVGVKIN EKTGKIPVTD EEQTNVPYIY AIGDILEGKL ELTPVAIQAG 
RLLAQRLYGG STVKCDYDNV PTTVFTPLEY GCCGLSEEKA VEKFGEENIE 
VYHSFFWPLE WTVPSRDNNK CYAKVICNLK DNERVVGFHV LGPNAGEVTQ 
GFAAALKCGL TKQQLDSTIG IHPVCAEIFT TLSVTKRSGG DILQSG'''C'''UG 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:528.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00450 Selenocompound metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05200 Pathways in cancer] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05225 Hepatocellular carcinoma] 

==Experimental Evidence==
:Cys Modification Assay, Mass Spectrometry, Western Blot

==Reference==
# Fang J, Lu J, Holmgren A. '''Thioredoxin reductase is irreversibly modified by curcumin a novel molecular mechanism for its anticancer activity[J].''' Journal of biological chemistry, 2005, 280(26): 25284-25290. [https://www.ncbi.nlm.nih.gov/pubmed/?term=15879598 15879598] 

[[Category:Targets|Targets]]
[[Category:Rattus norvegicus|Rattus norvegicus]]
[[Category:Redox protein|Redox protein]]
[[Category:Class-I pyridine nucleotide-disulfide oxidoreductase family|Class-I pyridine nucleotide-disulfide oxidoreductase family]]
[[Category:Selenocompound metabolism|Selenocompound metabolism]]
[[Category:Pathways in cancer|Pathways in cancer]]
[[Category:Hepatocellular carcinoma|Hepatocellular carcinoma]]

@@ Line 50: / Line 50: @@
 # Fang J, Lu J, Holmgren A. '''Thioredoxin reductase is irreversibly modified by curcumin a novel molecular mechanism for its anticancer activity[J].''' Journal of biological chemistry, 2005, 280(26): 25284-25290. [https://www.ncbi.nlm.nih.gov/pubmed/?term=15879598 15879598]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Rattus norvegicus|Rattus norvegicus]]
+[[Category:Rattus norvegicus]]
-[[Category:Redox protein|Redox protein]]
+[[Category:Redox protein]]
-[[Category:Class-I pyridine nucleotide-disulfide oxidoreductase family|Class-I pyridine nucleotide-disulfide oxidoreductase family]]
+[[Category:Class-I pyridine nucleotide-disulfide oxidoreductase family]]
-[[Category:Selenocompound metabolism|Selenocompound metabolism]]
+[[Category:Selenocompound metabolism]]
-[[Category:Pathways in cancer|Pathways in cancer]]
+[[Category:Pathways in cancer]]
-[[Category:Hepatocellular carcinoma|Hepatocellular carcinoma]]
+[[Category:Hepatocellular carcinoma]]

@@ Line 11: / Line 11: @@
 ===Cys Function & Property===
-Cys497 is very close to the active site of His472 in space.<br/>
+Cys497 is close to the active site of His472 in space.<br/>
 * Hydrophobic property:

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/family/PF02852 Pyridine nucleotide-disulphide oxidoreductase]<br/>Class-I pyridine nucleotide-disulfide oxidoreductase family|[[:Category:Thioredoxin reductase TR1 (Rattus norvegicus)|Ligand list]]|Redox protein}}
+{{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/family/PF02852 Pyridine nucleotide-disulphide oxidoreductase],<br/>Class-I pyridine nucleotide-disulfide oxidoreductase family|[[:Category:Thioredoxin reductase TR1 (Rattus norvegicus)|Ligand list]]|Redox protein}}
 ==Summary==

Thioredoxin reductase TR1 (Rattus norvegicus) - Revision history

Wu3857: /* Reference */

Wu3857: /* Cys Function & Property */

Wu3857 at 02:22, 4 August 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/fa..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Txnrd1, Trxr1|[https://www.uniprot.org/uniprot/O89049 O89049]|Rattus norvegicus|Cys497|[http://pfam.xfam.org/fa..."