Sorbitol dehydrogenase - Revision history

Wu3857: /* Reference */

2019-08-19T23:16:08Z

‎Reference

Wu3857 at 02:02, 18 August 2019

2019-08-18T02:02:42Z

Wu3857: /* Protein Function */

2019-08-18T02:01:50Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-18T02:01:20Z

‎Protein Function

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cy..."

2019-08-18T02:00:21Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cy..."

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{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cys130|[http://pfam.xfam.org/family/PF00107 Zinc-binding dehydrogenase], Zinc-containing alcohol dehydrogenase family|[[:Category:Sorbitol dehydrogenase|Ligand list]]|Transcription Related}}

==Summary==

===Protein Function ===
Sorbitol dehydrogenase is an enzyme include in carbohydrate metabolism converting sorbitol, the sugar alcohol form of glucose, into fructose. Together with aldose reductase, it provides a way for the body to produce fructose from glucose without using ATP. 
Sorbitol dehydrogenase uses NAD+ as a cofactor; its reaction is: 
sorbitol + NAD+ --> fructose + NADH + H+. 
A zinc ion is also involved in catalysis. Organs that use it most frequently include the liver and seminal vesicle; it is found in all kinds of organisms from bacteria to humans. A secondary use is the metabolism of dietary sorbitol, though sorbitol is known not to be absorbed as well in the intestine as its related compounds glucose and fructose, and is usually found in quite small amounts in the diet (except when used as an artificial sweetener). (From Wikipedia) 
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. (From Uniprot) 

===Cys Function & Property===
Cys45 is the metal binding site of Sord. And two regions, residues 90–110 and 120–140, are very important for the stability and binding between subunits in mammalian SDHs. (PMID: 22752181, 17952367) 

* Hydrophobic property:
:[[File:565-hydro.png||600px]]
* SASA：
:Cys45: Unknown 
:Cys120: Unknown 

==Protein Sequence==

MAAPAKGENL SLVVHGPGDI RLENYPIPEL GPNDVLLKMH SVGI'''C'''GSDVH 
YWEHGRIGDF VVKKPMVLGH EAAGTVTKVG PMVKHLKPGD RVAIEPGVPR 
EIDEFCKIGR YNLTPSIFFC ATPPDDGNL'''C''' RFYKHSADFC YKLPDSVTFE 
EGALIEPLSV GIYACRRGSV SLGNKVLVCG AGPIGIVTLL VAKAMGASQV 
VVIDLSASRL AKAKEVGADF TIQVAKETPH DIAKKVESVL GSKPEVTIEC 
TGAESSVQTG IYATHSGGTL VVVGMGPEMI NLPLVHAAVR EVDIKGVFRY 
CNTWPMAVSM LASKTLNVKP LVTHRFPLEK AVEAFETAKK GLGLKVMIKC 
DPNDQNP 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown 

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00040 Pentose and glucuronate interconversions] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00051 Fructose and mannose metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] 

==Experimental Evidence==
:MALDI-TOF/MS, Cys Modification Assay

==Reference==
# Kanda H, Toyama T, Shinohara-Kanda A, et al. '''S-Mercuration of rat sorbitol dehydrogenase by methylmercury causes its aggregation and the release of the zinc ion from the active site[J].''' Archives of toxicology, 2012, 86(11): 1693-1702. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22752181 22752181] 

[[Category:Targets|Targets]]
[[Category:Rattus norvegicus|Rattus norvegicus]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:Zinc-containing alcohol dehydrogenase family|Zinc-containing alcohol dehydrogenase family]]
[[Category:Pentose and glucuronate interconversions|Pentose and glucuronate interconversions]]
[[Category:Fructose and mannose metabolism|Fructose and mannose metabolism]]
[[Category:Metabolic pathways|Metabolic pathways]]

@@ Line 52: / Line 52: @@
 # Kanda H, Toyama T, Shinohara-Kanda A, et al. '''S-Mercuration of rat sorbitol dehydrogenase by methylmercury causes its aggregation and the release of the zinc ion from the active site[J].''' Archives of toxicology, 2012, 86(11): 1693-1702. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22752181 22752181]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Rattus norvegicus|Rattus norvegicus]]
+[[Category:Rattus norvegicus]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Metabolic enzyme]]
-[[Category:Zinc-containing alcohol dehydrogenase family|Zinc-containing alcohol dehydrogenase family]]
+[[Category:Zinc-containing alcohol dehydrogenase family]]
-[[Category:Pentose and glucuronate interconversions|Pentose and glucuronate interconversions]]
+[[Category:Pentose and glucuronate interconversions]]
-[[Category:Fructose and mannose metabolism|Fructose and mannose metabolism]]
+[[Category:Fructose and mannose metabolism]]
-[[Category:Metabolic pathways|Metabolic pathways]]
+[[Category:Metabolic pathways]]

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cys130|[http://pfam.xfam.org/family/PF00107 Zinc-binding dehydrogenase],<br/>Zinc-containing alcohol dehydrogenase family|[[:Category:Sorbitol dehydrogenase|Ligand list]]|Transcription Related}}
+{{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cys130|[http://pfam.xfam.org/family/PF00107 Zinc-binding dehydrogenase],<br/>Zinc-containing alcohol dehydrogenase family|[[:Category:Sorbitol dehydrogenase|Ligand list]]|Metabolic enzyme}}
 ==Summary==

@@ Line 8: / Line 8: @@
 ===Protein Function ===
 Sorbitol dehydrogenase is an enzyme include in carbohydrate metabolism converting sorbitol, the sugar alcohol form of glucose, into fructose. Together with aldose reductase, it provides a way for the body to produce fructose from glucose without using ATP. <br/>
-Sorbitol dehydrogenase uses NAD+ as a cofactor; its reaction is:<br/>
+Sorbitol dehydrogenase uses NAD<sup>+</sup> as a cofactor; its reaction is:<br/>
-<div align="center">Sorbitol + NAD+ --> fructose + NADH + H+.</div>
+<div align="center">Sorbitol + NAD<sup>+</sup> --> fructose + NADH + H<sup>+</sup>.</div>
 A zinc ion is also involved in catalysis. Organs that use it most frequently include the liver and seminal vesicle; it is found in all kinds of organisms from bacteria to humans. A secondary use is the metabolism of dietary sorbitol, though sorbitol is known not to be absorbed as well in the intestine as its related compounds glucose and fructose, and is usually found in quite small amounts in the diet (except when used as an artificial sweetener). (From Wikipedia) <br/>
 Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. (From Uniprot) <br/>

@@ Line 9: / Line 9: @@
 Sorbitol dehydrogenase is an enzyme include in carbohydrate metabolism converting sorbitol, the sugar alcohol form of glucose, into fructose. Together with aldose reductase, it provides a way for the body to produce fructose from glucose without using ATP. <br/>
 Sorbitol dehydrogenase uses NAD+ as a cofactor; its reaction is:<br/>
-sorbitol + NAD+ --> fructose + NADH + H+.<br/>
+<div align="center">Sorbitol + NAD+ --> fructose + NADH + H+.</div>
 A zinc ion is also involved in catalysis. Organs that use it most frequently include the liver and seminal vesicle; it is found in all kinds of organisms from bacteria to humans. A secondary use is the metabolism of dietary sorbitol, though sorbitol is known not to be absorbed as well in the intestine as its related compounds glucose and fructose, and is usually found in quite small amounts in the diet (except when used as an artificial sweetener). (From Wikipedia) <br/>
 Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. (From Uniprot) <br/>

Sorbitol dehydrogenase - Revision history

Wu3857: /* Reference */

Wu3857 at 02:02, 18 August 2019

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cy..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Sord, SDH, L-iditol 2-dehydrogenase|[https://www.uniprot.org/uniprot/P27867 P27867]|Rattus norvegicus|Cys45, Cy..."