Sodium/bile acid cotransporter - Revision history

Wu3857: /* Summary */

2019-08-22T03:43:07Z

‎Summary

Wu3857: /* Cys Function & Property */

2019-08-22T03:42:34Z

‎Cys Function & Property

Wu3857: /* Summary */

2019-08-22T03:38:39Z

‎Summary

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|SLC10A1|[https://www.uniprot.org/uniprot/Q14973 Q14973]|Homo sapiens|Cys96, Cys266|[http://pfam.xfam.org/family..."

2019-08-22T03:37:54Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|SLC10A1|[https://www.uniprot.org/uniprot/Q14973 Q14973]|Homo sapiens|Cys96, Cys266|[http://pfam.xfam.org/family..."

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{{#invoke:InfoboxforTarget|run|SLC10A1|[https://www.uniprot.org/uniprot/Q14973 Q14973]|Homo sapiens|Cys96, Cys266|[http://pfam.xfam.org/family/PF01758 Bile acid:sodium symporter family]|[[:Category:Sodium/bile acid cotransporter|Ligand list]]|Transporter}}
==Summary==

===Protein Function ===
The Na+-dependent taurocholate (TC) cotransporting polypeptide (NTCP/Ntcp) is the major sinusoidal bile salt transporter of hepatocytes. Ntcp mediates the transport of conjugated bile salts such as TC and taurochenodeoxycholate in a Na+-dependent fashion using the Na+ gradient produced by the Na+-K+-ATPase. (PMID: 23886862) 
:[[File:583-function.png|center|600px]]
<div align="center">Distribution of cysteine residues</div>

===Cys Function & Property===
Ntcp has 8 cysteines, and one of these, Cys266, is highly conserved not only across several species but also with the NTCP/Ntcp homolog, apical Na+-dependent bile acid transporter (Asbt). 
Some three-dimensional homology model for human NTCP predicts that Cys96 lies in a possible permeation pathway for Na+. And S-nitrosylation of Cys96 may result in steric hindrance that blocks the permeation pathway for Na+ and thereby inhibits Ntcp function. 

* Hydrophobic property:
:[[File:583-hydro.png||600px]]
* SASA：
:Unknown

==Protein Sequence==

MEAHNASAPF NFTLPPNFGK RPTDLALSVI LVFMLFFIML SLGCTMEFSK 
IKAHLWKPKG LAIALVAQYG IMPLTAFVLG KVFRLKNIEA LAILV'''C'''GCSP 
GGNLSNVFSL AMKGDMNLSI VMTTCSTFCA LGMMPLLLYI YSRGIYDGDL 
KDKVPYKGIV ISLVLVLIPC TIGIVLKSKR PQYMRYVIKG GMIIILLCSV 
AVTVLSAINV GKSIMFAMTP LLIATSSLMP FIGFLLGYVL SALFCLNGRC 
RRTVSMETGC QNVQL'''C'''STIL NVAFPPEVIG PLFFFPLLYM IFQLGEGLLL 
IAIFWCYEKF KTPKDKTKMI YTAATTEETI PGALGNGTYK GEDCSPCTA 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04976 Bile secretion] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05161 Hepatitis B] 

==Experimental Evidence==
:Cys-directed Mutation, Homology Modeling

==Reference==
# Ramasamy U, Anwer M S, Schonhoff C M. '''Cysteine 96 of Ntcp is responsible for NO-mediated inhibition of taurocholate uptake[J].''' American Journal of Physiology-Gastrointestinal and Liver Physiology, 2013, 305(7): G513-G519. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23886862 23886862] 

[[Category:Targets]]
[[Category:Homo sapiens]]
[[Category:Transporter]]
[[Category:Bile acid:sodium symporter family]]
[[Category:Bile secretion]]
[[Category:Hepatitis B]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 The Na<sup>+</sup>-dependent taurocholate (TC) cotransporting polypeptide (NTCP/Ntcp) is the major sinusoidal bile salt transporter of hepatocytes. Ntcp mediates the transport of conjugated bile salts such as TC and taurochenodeoxycholate in a Na<sup>+</sup>-dependent fashion using the Na<sup>+</sup> gradient produced by the Na<sup>+</sup>-K<sup>+</sup>-ATPase. (PMID: 23886862) <br/>
-:[[File:583-function.png|center|400px]]
+[[File:583-function.png|center|400px]]
 <div align="center">Distribution of cysteine residues</div>

@@ Line 11: / Line 11: @@
 ===Cys Function & Property===
-Ntcp has 8 cysteines, and one of these, Cys266, is highly conserved not only across several species but also with the NTCP/Ntcp homolog, apical Na+-dependent bile acid transporter (Asbt). <br/>
+Ntcp has 8 cysteines, and one of these, Cys266, is highly conserved not only across several species but also with the NTCP/Ntcp homolog, apical Na<sup>+</sup>-dependent bile acid transporter (Asbt). <br/>
 Some three-dimensional homology model for human NTCP predicts that Cys96 lies in a possible permeation pathway for Na<sup>+</sup>. And S-nitrosylation of Cys96 may result in steric hindrance that blocks the permeation pathway for Na<sup>+</sup> and thereby inhibits Ntcp function. <br/>

Sodium/bile acid cotransporter - Revision history

Wu3857: /* Summary */

Wu3857: /* Cys Function & Property */

Wu3857: /* Summary */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|SLC10A1|[https://www.uniprot.org/uniprot/Q14973 Q14973]|Homo sapiens|Cys96, Cys266|[http://pfam.xfam.org/family..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|SLC10A1|[https://www.uniprot.org/uniprot/Q14973 Q14973]|Homo sapiens|Cys96, Cys266|[http://pfam.xfam.org/family..."