Wu3857: /* Reference */

2019-08-19T23:13:13Z

‎Reference

Wu3857 at 02:46, 31 July 2019

2019-07-31T02:46:39Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://p..."

2019-07-31T02:38:33Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://p..."

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{{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://pfam.xfam.org/family/PF00615 Regulator of G protein signaling domain]|[[:Category:Regulator of G-protein signaling 4|Ligand list]]|ATPase/GTPase}}
==Summary==

===Protein Function ===
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. (From Uniprot) 
Regulator of G protein signaling 4 also known as RGP4 is a protein that in humans is encoded by the RGS4 gene. RGP4 regulates G protein signaling. Regulator of G protein signalling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain which conveys GAP activity.#Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulates signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. (From Wiki)
 

===Cys Function & Property===
To be added. 

* Hydrophobic property:
:[[File:507-hydro.png||600px]]
* SASA：
:Unknown 

==Protein Sequence==

MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHNSSHN KKDKVVICQR 
VSQEEVKKWA ESLENLISHE '''C'''GLAAFKAFL KSEYSEENID FWISCEEYKK 
IKSPSKLSPK AKKIYNEFIS VQATKEVNLD S'''C'''TREETSRN MLEPTIT'''C'''FD 
EAQKKIFNLM EKDSYRRFLK SRFYLDLVNP SS'''C'''GAEKQKG AKSSADCASL 
VPQCA 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown 

==Related Pathway==
:Unknown 

==Experimental Evidence==
:Cys-directed Mutation, Surface Plasmon Resonance-based Binding Assay, MS, Western Blot

==Reference==
# Monroy C A, Doorn J A, Roman D L. '''Modification and functional inhibition of regulator of G-protein signaling 4 (RGS4) by 4-hydroxy-2-nonenal[J].''' Chemical research in toxicology, 2013, 26(12): 1832-1839. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24229325 24229325] 
# Kimple A J, Willard F S, Giguère P M, et al. '''The RGS protein inhibitor CCG-4986 is a covalent modifier of the RGS4 Gα-interaction face[J].''' Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 2007, 1774(9): 1213-1220. [https://www.ncbi.nlm.nih.gov/pubmed/?term=17660054 17660054] 
# Roof R A, Roman D L, Clements S T, et al. '''A covalent peptide inhibitor of RGS4 identified in a focused one-bead, one compound library screen[J].''' BMC pharmacology, 2009, 9(1): 9. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19463173 19463173] 
# Roman D L, Ota S, Neubig R R. '''Polyplexed flow cytometry protein interaction assay: a novel high-throughput screening paradigm for RGS protein inhibitors[J].''' Journal of biomolecular screening, 2009, 14(6): 610-619. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19531661 19531661] 
# Storaska A J, Mei J P, Wu M, et al. '''Reversible inhibitors of regulators of G-protein signaling identified in a high-throughput cell-based calcium signaling assay[J].''' Cellular signalling, 2013, 25(12): 2848-2855. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24041654 24041654] 
# Blazer L L, Roman D L, Chung A, et al. '''Reversible, allosteric small-molecule inhibitors of regulator of G protein signaling proteins[J].''' Molecular pharmacology, 2010, 78(3): 524-533. [https://www.ncbi.nlm.nih.gov/pubmed/?term=20571077 20571077] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:ATPase/GTPase|ATPase/GTPase]]
[[Category:Regulator of G protein signaling domain|Regulator of G protein signaling domain]]

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 # Blazer L L, Roman D L, Chung A, et al. '''Reversible, allosteric small-molecule inhibitors of regulator of G protein signaling proteins[J].''' Molecular pharmacology, 2010, 78(3): 524-533. [https://www.ncbi.nlm.nih.gov/pubmed/?term=20571077 20571077]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:ATPase/GTPase|ATPase/GTPase]]
+[[Category:ATPase/GTPase]]
-[[Category:Regulator of G protein signaling domain|Regulator of G protein signaling domain]]
+[[Category:Regulator of G protein signaling domain]]

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-{{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://pfam.xfam.org/family/PF00615 Regulator of G protein signaling domain]|[[:Category:Regulator of G-protein signaling 4|Ligand list]]|ATPase/GTPase}}
+{{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132,<br/>Cys148, Cys183|[http://pfam.xfam.org/family/PF00615 Regulator of G protein signaling domain]|[[:Category:Regulator of G-protein signaling 4|Ligand list]]|ATPase/GTPase}}
 ==Summary==

Regulator of G-protein signaling 4 - Revision history

Wu3857: /* Reference */

Wu3857 at 02:46, 31 July 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://p..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|RGP4, RGS4|[https://www.uniprot.org/uniprot/P49798 P49798]|Homo sapiens|Cys71, Cys132, Cys148, Cys183|[http://p..."