RNA demethylase ALKBH5 - Revision history

Wu3857 at 20:09, 26 May 2020

2020-05-26T20:09:24Z

Wu3857 at 17:20, 25 May 2020

2020-05-25T17:20:31Z

Wu3857 at 17:20, 25 May 2020

2020-05-25T17:20:21Z

Wu3857 at 17:20, 25 May 2020

2020-05-25T17:20:11Z

Wu3857 at 17:19, 25 May 2020

2020-05-25T17:19:51Z

Wu3857 at 17:14, 25 May 2020

2020-05-25T17:14:55Z

Wu3857 at 17:13, 25 May 2020

2020-05-25T17:13:45Z

Wu3857: /* Reference */

2020-05-25T17:12:04Z

‎Reference

Wu3857: /* Reference */

2020-05-25T17:11:41Z

‎Reference

Wu3857 at 17:11, 25 May 2020

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 ==Reference==
 # Aik W S, Scotti J S, Choi H, et al. '''Structure of human RNA N 6-methyladenine demethylase ALKBH5 provides insights into its mechanisms of nucleic acid recognition and demethylation[J].''' Nucleic acids research, 2014, 42(7): 4741-4754. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24489119 24489119]<br/>
 [[Category:2OG-Fe(II) oxygenase superfamily]]
 [[Category:Alkb family]]

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 ===Protein Function ===
-ALKBH5 belongs to the conserved AlkB family of non-heme Fe(II)/alpha-KG-dependent dioxygenases that repair N-alkylated nucleobases by oxidative demethylation. It could catalyze the demethylation of m6A of ssRNA. N6-Methyladenosine (m6A) is the most prevalent and abundant internal methylated nucleoside in mammalian mRNA and is also found in the RNA of plants and viruses. Mapping of m6A in human and mouse RNA identified that m6A modification happens mainly within the consensus sequence (G/A)(G/A)m6AC(A/C/U) in a non-stoichiometric manner with only three to five m6A sites observed per mRNA molecule.m6A sites are enriched near stop codons and in the 3'-UTR of mRNA. <br/><br/>
+ALKBH5 belongs to the conserved AlkB family of non-heme Fe(II)/alpha-KG-dependent dioxygenases that repair N-alkylated nucleobases by oxidative demethylation. It could catalyze the demethylation of m6A of ssRNA. N6-Methyladenosine (m6A) is the most prevalent and abundant internal methylated nucleoside in mammalian mRNA and is also found in the RNA of plants and viruses. Mapping of m6A in human and mouse RNA identified that m6A modification happens mainly within the consensus sequence (G/A)(G/A)m6AC(A/C/U) in a non-stoichiometric manner with only three to five m6A sites observed per mRNA molecule.m6A sites are enriched near stop codons and in the 3'-UTR of mRNA. <br/>
 ALKBH5 is localized to the nucleus, has 2OG and iron-dependent activity and is upregulated under hypoxic conditions by the hypoxia-inducible factor (HIF) transcription factor pathway. ALKBH5 was observed to be highly expressed in the lung, followed by testis, pancreas, spleen and ovary. It was also reported that ALKBH5 localized to nuclear speckles and that decreased ALKBH5 levels affect mRNA export and processing, leading to altered spermatogenesis. <br/>

← Older revision		Revision as of 17:20, 25 May 2020
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	ALKBH5 is localized to the nucleus, has 2OG and iron-dependent activity and is upregulated under hypoxic conditions by the hypoxia-inducible factor (HIF) transcription factor pathway. ALKBH5 was observed to be highly expressed in the lung, followed by testis, pancreas, spleen and ovary. It was also reported that ALKBH5 localized to nuclear speckles and that decreased ALKBH5 levels affect mRNA export and processing, leading to altered spermatogenesis. <br/>		ALKBH5 is localized to the nucleus, has 2OG and iron-dependent activity and is upregulated under hypoxic conditions by the hypoxia-inducible factor (HIF) transcription factor pathway. ALKBH5 was observed to be highly expressed in the lung, followed by testis, pancreas, spleen and ovary. It was also reported that ALKBH5 localized to nuclear speckles and that decreased ALKBH5 levels affect mRNA export and processing, leading to altered spermatogenesis. <br/>
−

	===Cys Function & Property===		===Cys Function & Property===

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 ===Protein Function ===
-ALKBH5 belongs to the conserved AlkB family of non-heme Fe(II)/alpha-KG-dependent dioxygenases that repair N-alkylated nucleobases by oxidative demethylation. It could catalyze the demethylation of m6A of ssRNA. N6-Methyladenosine (m6A) is the most prevalent and abundant internal methylated nucleoside in mammalian mRNA and is also found in the RNA of plants and viruses. Mapping of m6A in human and mouse RNA identified that m6A modification happens mainly within the consensus sequence (G/A)(G/A)m6AC(A/C/U) in a non-stoichiometric manner with only three to five m6A sites observed per mRNA molecule.m6A sites are enriched near stop codons and in the 3'-UTR ofmRNA <br/>
+ALKBH5 belongs to the conserved AlkB family of non-heme Fe(II)/alpha-KG-dependent dioxygenases that repair N-alkylated nucleobases by oxidative demethylation. It could catalyze the demethylation of m6A of ssRNA. N6-Methyladenosine (m6A) is the most prevalent and abundant internal methylated nucleoside in mammalian mRNA and is also found in the RNA of plants and viruses. Mapping of m6A in human and mouse RNA identified that m6A modification happens mainly within the consensus sequence (G/A)(G/A)m6AC(A/C/U) in a non-stoichiometric manner with only three to five m6A sites observed per mRNA molecule.m6A sites are enriched near stop codons and in the 3'-UTR of mRNA. <br/><br/>
 ALKBH5 is localized to the nucleus, has 2OG and iron-dependent activity and is upregulated under hypoxic conditions by the hypoxia-inducible factor (HIF) transcription factor pathway. ALKBH5 was observed to be highly expressed in the lung, followed by testis, pancreas, spleen and ovary. It was also reported that ALKBH5 localized to nuclear speckles and that decreased ALKBH5 levels affect mRNA export and processing, leading to altered spermatogenesis. <br/>

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 ===Protein Function ===
-Caspase-2 proteolytically cleaves other proteins. It belongs to a family of cysteine proteases called caspases that cleave proteins only at an amino acid following an aspartic acid residue. Within this family, caspase-2 is part of the Ich-1 subfamily. It is one of the most conserved caspases in different species of animal. Caspase-2 has a similar amino acid sequence to initiator caspases, including caspase 1, caspase-4, caspase-5, and caspase-9. It is produced as a zymogen, which contains a long pro-domain that is similar to that of caspase-9 and contains a protein interaction domain known as a CARD domain. Pro-caspase-2 contains two subunits, p19 and p12. (From Wikipedia) <br/>
+ALKBH5 belongs to the conserved AlkB family of non-heme Fe(II)/alpha-KG-dependent dioxygenases that repair N-alkylated nucleobases by oxidative demethylation. It could catalyze the demethylation of m6A of ssRNA. N6-Methyladenosine (m6A) is the most prevalent and abundant internal methylated nucleoside in mammalian mRNA and is also found in the RNA of plants and viruses. Mapping of m6A in human and mouse RNA identified that m6A modification happens mainly within the consensus sequence (G/A)(G/A)m6AC(A/C/U) in a non-stoichiometric manner with only three to five m6A sites observed per mRNA molecule.m6A sites are enriched near stop codons and in the 3'-UTR ofmRNA <br/>
 ===Cys Function & Property===

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 * Hydrophobic property:
-:[[File:ALKBH5-hydro.png||600px]]
 * SASA:
 :Unkown<br/>
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 *Protein structure:
-[[File:ALKBH5.png|center|800px]]
 ==Related Pathway==

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 :[[File:ALKBH5-hydro.png||600px]]
 * SASA:
-:Cys200: ? A^2<br/>
+:Unkown<br/>
 ==Protein Sequence==

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 ==Reference==
-# Aik W S, Scotti J S, Choi H, et al. '''Structure of human RNA N 6-methyladenine demethylase ALKBH5 provides insights into its mechanisms of nucleic acid recognition and demethylation[J].''' Nucleic acids research, 2014, 42(7): 4741-4754. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24489119 24489119] <br/>
+# Aik W S, Scotti J S, Choi H, et al. '''Structure of human RNA N 6-methyladenine demethylase ALKBH5 provides insights into its mechanisms of nucleic acid recognition and demethylation[J].''' Nucleic acids research, 2014, 42(7): 4741-4754. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24489119 24489119]<br/>
 [[Category:Targets]]
 [[Category:Homo sapiens]]