Polypeptide deformylase - Revision history

Wu3857: /* Reference */

2019-08-19T23:10:33Z

‎Reference

Wu3857 at 20:45, 29 July 2019

2019-07-29T20:45:51Z

Wu3857: /* Cys Function & Property */

2019-07-29T03:23:14Z

‎Cys Function & Property

Wu3857: /* Cys Function & Property */

2019-07-29T03:21:54Z

‎Cys Function & Property

Wu3857: /* Cys Function & Property */

2019-07-29T03:21:44Z

‎Cys Function & Property

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327..."

2019-07-29T03:21:30Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327..."

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{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327 Polypeptide deformylase family]|[[:Category:Polypeptide deformylase|Ligand list]]}}
==Summary==

===Protein Function ===
Removal of the formyl moiety on methionine of nascent proteins by the metalloprotease peptide deformylase (PDF) is a necessary activity for prokaryotic cell viability. This activity was not believed to be important in eukaryotic cells until recently, because nuclear encoded proteins are not N-formylated. However, in eukaryotes, mitochondrial protein synthesis may involve the formylation and deformylation of proteins, as evidenced by the presence of the enzymatic machinery to perform these activities in mammals and plants, among other eukaryotes. A human peptide deformylase (HsPDF) was recently shown to be present in mitochondria. 
The physiologic role of PDF in mammalian cells is unclear. The mitochondrial localization of HsPDF, and the N-terminal Met-formylation of mammalian mitochondrial translation products for translation initiation point at the 13 proteins encoded by the mitochondrial genome as putative substrates of HsPDF.
 

===Cys Function & Property===
Cys172 is used to bind with metal, as shown below. 
[[File:494-function.png|center|800px]]

* Hydrophobic property:
:[[File:494-hydro.png||600px]]
* SASA：
:Cys172: 8.759 A^2

==Protein Sequence==

MARLWGALSL WPLWAAVPWG GAAAVGVRAC SSTAAPDGVE GPALRRSYWR 
HLRRLVLGPP EPPFSHVCQV GDPVLRGVAA PVERAQLGGP ELQRLTQRLV 
QVMRRRRCVG LSAPQLGVPR QVLALELPEA L'''C'''RECPPRQR ALRQMEPFPL 
RVFVNPSLRV LDSRLVTFPE GCESVAGFLA CVPRFQAVQI SGLDPNGEQV 
VWQASGWAAR IIQHEMDHLQ GCLFIDKMDS RTFTNVYWMK VND 


==Structural Information==
*Known structures with covalent ligands: 
:[https://www.rcsb.org/structure/3G5K 3G5K] 

*Protein structure:
[[File:494.png|center|800px]]

==Related Pathway==
*Unknown 

==Experimental Evidence==
:Crystallography, Homologous Analysis of Sequence

==Reference==
# Escobar-Alvarez S, Goldgur Y, Yang G, et al. '''Structure and activity of human mitochondrial peptide deformylase, a novel cancer target[J].''' Journal of molecular biology, 2009, 387(5): 1211-1228. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19236878 19236878] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Polypeptide deformylase family|Polypeptide deformylase family]]

@@ Line 44: / Line 44: @@
 # Escobar-Alvarez S, Goldgur Y, Yang G, et al. '''Structure and activity of human mitochondrial peptide deformylase, a novel cancer target[J].''' Journal of molecular biology, 2009, 387(5): 1211-1228. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19236878 19236878]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Protease|Protease]]
+[[Category:Protease]]
-[[Category:Polypeptide deformylase family|Polypeptide deformylase family]]
+[[Category:Polypeptide deformylase family]]

@@ Line 11: / Line 11: @@
 ===Cys Function & Property===
-Cys172 is used to bind with metal, as shown below.<br/>
+Cys172 is used to bind with metal ion (Co<sup>2+</sup>), as shown below.<br/>
 [[File:494-function.png|center|500px]]

@@ Line 12: / Line 12: @@
 ===Cys Function & Property===
 Cys172 is used to bind with metal, as shown below.<br/>
-[[File:494-function.png|center|600px]]
+[[File:494-function.png|center|500px]]
 * Hydrophobic property:

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327 Polypeptide deformylase family]|[[:Category:Polypeptide deformylase|Ligand list]]}}
+{{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327 Polypeptide deformylase family]|[[:Category:Polypeptide deformylase|Ligand list]]|Protease}}
 ==Summary==
@@ Line 46: / Line 46: @@
 [[Category:Targets|Targets]]
 [[Category:Homo sapiens|Homo sapiens]]
 [[Category:Polypeptide deformylase family|Polypeptide deformylase family]]

Polypeptide deformylase - Revision history

Wu3857: /* Reference */

Wu3857 at 20:45, 29 July 2019

Wu3857: /* Cys Function & Property */

Wu3857: /* Cys Function & Property */

Wu3857: /* Cys Function & Property */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|PDF1A|[https://www.uniprot.org/uniprot/Q9HBH1 Q9HBH1]|Homo sapiens|Cys172|[http://pfam.xfam.org/family/PF01327..."