N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 - Revision history

Wu3857: /* Reference */

2019-08-19T23:07:33Z

‎Reference

Wu3857: /* Protein Function */

2019-08-15T02:41:38Z

‎Protein Function

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|DDAH2|[https://www.uniprot.org/uniprot/O95865 O95865]|Homo sapiens|Cys276|DDAH family|:Category:N(G),N(G)-dim..."

2019-08-15T02:41:26Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|DDAH2|[https://www.uniprot.org/uniprot/O95865 O95865]|Homo sapiens|Cys276|DDAH family|:Category:N(G),N(G)-dim..."

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{| align="left"
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{{#invoke:InfoboxforTarget|run|DDAH2|[https://www.uniprot.org/uniprot/O95865 O95865]|Homo sapiens|Cys276|DDAH family|[[:Category:N(G),N(G)-dimethylarginine dimethylaminohydrolase 2|Ligand list]]|Metabolic enzyme}}
==Summary==

===Protein Function ===
In the field of enzymology, a dimethylargininase, also known as a dimethylarginine dimethylaminohydrolase (DDAH), is an enzyme that catalyzes the chemical reaction: 
<div align="center">N-omega,N-omega'-methyl-L-arginine + H2O ↔ dimethylamine + L-citrulline</div> 
DDAH is an enzyme found in all mammalian cells. Two isoforms exist, DDAH I and DDAH II, with some differences in tissue distribution of the two isoforms. The enzyme degrades methylarginines, specifically asymmetric dimethylarginine (ADMA) and NG-monomethyl-L-arginine (MMA). 
The methylarginines ADMA and MMA inhibit the production of nitric oxide synthase. As such, DDAH is important in removing methylarginines, generated by protein degradation, from accumulating and inhibiting the generation of nitric oxide. (From Wikipedia) 
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. (From Uniprot) 

===Cys Function & Property===
Cys276 is one of the active site of DDAH1, which act as the nucleophile group in catalyze. 

* Hydrophobic property:
:[[File:559-hydro.png||600px]]
* SASA：
:Cys276: Unknown 

==Protein Sequence==

MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG 
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV 
DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE 
IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA 
VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ 
KLSDVTLVPV SCSELEKAGA GLSSL'''C'''LVLS TRPHS 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown 

==Related Pathway==
:Unknown 

==Experimental Evidence==
:Homologous Analysis of Sequence, Molecular Docking

==Reference==
# Wang Y, Monzingo A F, Hu S, et al. '''Developing dual and specific inhibitors of dimethylarginine dimethylaminohydrolase-1 and nitric oxide synthase: toward a targeted polypharmacology to control nitric oxide[J].''' Biochemistry, 2009, 48(36): 8624-8635. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19663506 19663506] 
# Linsky T W, Fast W. '''Discovery of structurally-diverse inhibitor scaffolds by high-throughput screening of a fragment library with dimethylarginine dimethylaminohydrolase[J].''' Bioorganic & medicinal chemistry, 2012, 20(18): 5550-5558. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22921743 22921743] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:DDAH family|DDAH family]]

@@ Line 47: / Line 47: @@
 # Linsky T W, Fast W. '''Discovery of structurally-diverse inhibitor scaffolds by high-throughput screening of a fragment library with dimethylarginine dimethylaminohydrolase[J].''' Bioorganic & medicinal chemistry, 2012, 20(18): 5550-5558. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22921743 22921743]<br/>
-[[Category:Targets|Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Targets]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Homo sapiens]]
-[[Category:DDAH family|DDAH family]]
+[[Category:Metabolic enzyme]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 In the field of enzymology, a dimethylargininase, also known as a dimethylarginine dimethylaminohydrolase (DDAH), is an enzyme that catalyzes the chemical reaction: <br/>
-<div align="center">N-omega,N-omega'-methyl-L-arginine + H2O ↔ dimethylamine + L-citrulline</div><br/>
+<div align="center">N-omega,N-omega'-methyl-L-arginine + H2O ↔ dimethylamine + L-citrulline</div>
 DDAH is an enzyme found in all mammalian cells. Two isoforms exist, DDAH I and DDAH II, with some differences in tissue distribution of the two isoforms. The enzyme degrades methylarginines, specifically asymmetric dimethylarginine (ADMA) and NG-monomethyl-L-arginine (MMA).<br/>
 The methylarginines ADMA and MMA inhibit the production of nitric oxide synthase. As such, DDAH is important in removing methylarginines, generated by protein degradation, from accumulating and inhibiting the generation of nitric oxide. (From Wikipedia)<br/>

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 - Revision history

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|DDAH2|[https://www.uniprot.org/uniprot/O95865 O95865]|Homo sapiens|Cys276|DDAH family|:Category:N(G),N(G)-dim..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|DDAH2|[https://www.uniprot.org/uniprot/O95865 O95865]|Homo sapiens|Cys276|DDAH family|:Category:N(G),N(G)-dim..."