Legumain - Revision history

Wu3857: /* Reference */

2019-08-19T23:05:28Z

‎Reference

Wu3857: /* Summary */

2019-08-18T18:47:59Z

‎Summary

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|LGMN, PRSC1|[https://www.uniprot.org/uniprot/Q99538 Q99538]|Homo sapiens|Cys189|[http://pfam.xfam.org/family/PF..."

2019-08-18T18:44:29Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|LGMN, PRSC1|[https://www.uniprot.org/uniprot/Q99538 Q99538]|Homo sapiens|Cys189|[http://pfam.xfam.org/family/PF..."

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{{#invoke:InfoboxforTarget|run|LGMN, PRSC1|[https://www.uniprot.org/uniprot/Q99538 Q99538]|Homo sapiens|Cys189|[http://pfam.xfam.org/family/PF01605 Peptidase C13 family]|[[:Category:Legumain|Ligand list]]|Protease}}
==Summary==

===Protein Function ===
Legumain is a cysteine protease from the C13 family of the CD clan of proteases (MEROPS). It uses a catalytic triad of Cysteine-Histidine-Asparagine in its active site to perform covalent proteolysis of its substrate. It catalyses the following chemical reaction: Hydrolysis of proteins and small molecule substrates at -Asn-Xaa- bonds. Both plant and animal legumains are most active in acidic environments. (From Wikipedia) 

===Cys Function & Property===
Cys189 is one of the active sites of Legumain. 

* Hydrophobic property:
:[[File:568-hydro.png||600px]]
* SASA：
:Cys189: 4.399 A^2 

==Protein Sequence==

MVWKVAVFLS VALGIGAVPI DDPEDGGKHW VVIVAGSNGW YNYRHQADAC 
HAYQIIHRNG IPDEQIVVMM YDDIAYSEDN PTPGIVINRP NGTDVYQGVP 
KDYTGEDVTP QNFLAVLRGD AEAVKGIGSG KVLKSGPQDH VFIYFTDHGS 
TGILVFPNED LHVKDLNETI HYMYKHKMYR KMVFYIEA'''C'''E SGSMMNHLPD 
NINVYATTAA NPRESSYACY YDEKRSTYLG DWYSVNWMED SDVEDLTKET 
LHKQYHLVKS HTNTSHVMQY GNKTISTMKV MQFQGMKRKA SSPVPLPPVT 
HLDLTPSPDV PLTIMKRKLM NTNDLEESRQ LTEEIQRHLD ARHLIEKSVR 
KIVSLLAASE AEVEQLLSER APLTGHSCYP EALLHFRTHC FNWHSPTYEY 
ALRHLYVLVN LCEKPYPLHR IKLSMDHVCL GHY 


==Structural Information==
*Known structure with covalent ligand: 
:[https://www.rcsb.org/structure/4AWB 4AWB], [https://www.rcsb.org/structure/4AW9 4AW9], [https://www.rcsb.org/structure/4AWA 4AWA] 

*Protein structure:
:[[File:568.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04142 Lysosome] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04612 Antigen processing and presentation] 

==Experimental Evidence==
:Crystallography

==Reference==
# Lee J, Bogyo M. '''Synthesis and evaluation of aza-peptidyl inhibitors of the lysosomal asparaginyl endopeptidase, legumain[J].''' Bioorganic & medicinal chemistry letters, 2012, 22(3): 1340-1343. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22243962 22243962] 
# Dall E, Brandstetter H. '''Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation[J].''' Proceedings of the National Academy of Sciences, 2013, 110(27): 10940-10945. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23776206 23776206] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Protease|Protease]]
[[Category:Peptidase C13 family|Peptidase C13 family]]
[[Category:Lysosome|Lysosome]]
[[Category:Antigen processing and presentation|Antigen processing and presentation]]

@@ Line 48: / Line 48: @@
 # Dall E, Brandstetter H. '''Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation[J].''' Proceedings of the National Academy of Sciences, 2013, 110(27): 10940-10945. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23776206 23776206]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Protease|Protease]]
+[[Category:Protease]]
-[[Category:Peptidase C13 family|Peptidase C13 family]]
+[[Category:Peptidase C13 family]]
-[[Category:Lysosome|Lysosome]]
+[[Category:Lysosome]]
-[[Category:Antigen processing and presentation|Antigen processing and presentation]]
+[[Category:Antigen processing and presentation]]

@@ Line 6: / Line 6: @@
 ===Protein Function ===
-Legumain is a cysteine protease from the C13 family of the CD clan of proteases (MEROPS). It uses a catalytic triad of Cysteine-Histidine-Asparagine in its active site to perform covalent proteolysis of its substrate. It catalyses the following chemical reaction: Hydrolysis of proteins and small molecule substrates at -Asn-Xaa- bonds. Both plant and animal legumains are most active in acidic environments. (From Wikipedia) <br/>
+Legumain is a cysteine protease from the C13 family of the CD clan of proteases (MEROPS). It uses a catalytic triad of Cysteine-Histidine-Asparagine in its active site to perform covalent proteolysis of its substrate.<br/>
 ===Cys Function & Property===

Legumain - Revision history

Wu3857: /* Reference */

Wu3857: /* Summary */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|LGMN, PRSC1|[https://www.uniprot.org/uniprot/Q99538 Q99538]|Homo sapiens|Cys189|[http://pfam.xfam.org/family/PF..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|LGMN, PRSC1|[https://www.uniprot.org/uniprot/Q99538 Q99538]|Homo sapiens|Cys189|[http://pfam.xfam.org/family/PF..."