Laforin - Revision history

Wu3857: /* Reference */

2019-08-19T23:04:56Z

‎Reference

Wu3857 at 00:39, 10 August 2019

2019-08-10T00:39:32Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http:..."

2019-08-10T00:39:17Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http:..."

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{| align="left"
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{{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http://pfam.xfam.org/family/PF00782 Dual specificity phosphatase, catalytic domain], Protein-tyrosine phosphatase family|[[:Category:Laforin|Ligand list]]|Phosphatase, Post-translational Modification}}

==Summary==

===Protein Function ===
Plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Dephosphorylates phosphotyrosine and synthetic substrates, such as para-nitrophenylphosphate (pNPP), and has low activity with phosphoserine and phosphothreonine substrates (in vitro). Has been shown to dephosphorylate MAPT. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. (From Uniprot) 

===Cys Function & Property===
Cys266 is the active site of Laforin, act as the phosphocysteine intermediate. 

* Hydrophobic property:
:[[File:547-hydro.png||600px]]
* SASA：
:Cys266: Unknown

==Protein Sequence==

MRFRFGVVVP PAVAGARPEL LVVGSRPELG RWEPRGAVRL RPAGTAAGDG 
ALALQEPGLW LGEVELAAEE AAQDGAEPGR VDTFWYKFLK REPGGELSWE 
GNGPHHDRCC TYNENNLVDG VYCLPIGHWI EATGHTNEMK HTTDFYFNIA 
GHQAMHYSRI LPNIWLGSCP RQVEHVTIKL KHELGITAVM NFQTEWDIVQ 
NSSGCNRYPE PMTPDTMIKL YREEGLAYIW MPTPDMSTEG RVQMLPQAVC 
LLHALLEKGH IVYVH'''C'''NAGV GRSTAAVCGW LQYVMGWNLR KVQYFLMAKR 
PAVYIDEEAL ARAQEDFFQK FGKVRSSVCS L 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:547.png|center|800px]]

==Related Pathway==
:Unknown 

==Experimental Evidence==
:LC-MS/MS, Achromobacter Protease I Digest

==Reference==
# Ueda K, Usui T, Nakayama H, et al. '''4‐Isoavenaciolide covalently binds and inhibits VHR, a dual‐specificity phosphatase[J].''' FEBS letters, 2002, 525(1-3): 48-52. [https://www.ncbi.nlm.nih.gov/pubmed/?term=12163160 12163160] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Phosphatase|Phosphatase]]
[[Category:Post-translational Modification|Post-translational Modification]]
[[Category:Protein-tyrosine phosphatase family|Protein-tyrosine phosphatase family]]

@@ Line 44: / Line 44: @@
 # Ueda K, Usui T, Nakayama H, et al. '''4‐Isoavenaciolide covalently binds and inhibits VHR, a dual‐specificity phosphatase[J].''' FEBS letters, 2002, 525(1-3): 48-52. [https://www.ncbi.nlm.nih.gov/pubmed/?term=12163160 12163160]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Phosphatase|Phosphatase]]
+[[Category:Phosphatase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:Protein-tyrosine phosphatase family|Protein-tyrosine phosphatase family]]
+[[Category:Protein-tyrosine phosphatase family]]

@@ Line 2: / Line 2: @@
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-{{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http://pfam.xfam.org/family/PF00782 Dual specificity phosphatase, catalytic domain],<br/>Protein-tyrosine phosphatase family|[[:Category:Laforin|Ligand list]]|Phosphatase,<br/>Post-translational Modification}}
+{{#invoke:InfoboxforTarget|run|LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http://pfam.xfam.org/family/PF00782 Dual specificity phosphatase, catalytic domain],<br/>Protein-tyrosine phosphatase family|[[:Category:Laforin|Ligand list]]|Phosphatase,<br/>Post-translational Modification}}
 ==Summary==

Laforin - Revision history

Wu3857: /* Reference */

Wu3857 at 00:39, 10 August 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http:..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|Glucan phosphatase, LAFPTPase, EPM2A|[https://www.uniprot.org/uniprot/O95278 O95278]|Homo sapiens|Cys266|[http:..."