Inosine-5'-monophosphate dehydrogenase 2 - Revision history

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2019-08-19T23:02:23Z

‎Reference

Wu3857: /* Reference */

2019-07-30T01:44:04Z

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Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|IMPDH2|[https://www.uniprot.org/uniprot/P12268 P12268]|Homo sapiens|Cys331|[http://pfam.xfam.org/family/PF00478..."

2019-07-30T01:41:54Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|IMPDH2|[https://www.uniprot.org/uniprot/P12268 P12268]|Homo sapiens|Cys331|[http://pfam.xfam.org/family/PF00478..."

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{{#invoke:InfoboxforTarget|run|IMPDH2|[https://www.uniprot.org/uniprot/P12268 P12268]|Homo sapiens|Cys331|[http://pfam.xfam.org/family/PF00478 IMPDH/GMPR family]|[[:Category:Inosine-5'-monophosphate dehydrogenase 2|Ligand list]]|Metabolic enzyme}}
==Summary==

===Protein Function ===
IMPDH2 catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. It could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. (From Uniprot) 

===Cys Function & Property===
Cys331 is one of the active sites of IMPDH2, acts as the thioimidate intermediate during the catalytic process. 

* Hydrophobic property:
:[[File:498-hydro.png||600px]]
* SASA：
:Cys331: 60.209 A^2

==Protein Sequence==

MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD 
LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ 
ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM 
GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI 
LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI 
GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI 
GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI '''C'''ITQEVLACG RPQATAVYKV 
SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY 
FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK 
GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE 
GGVHSLHSYE KRLF 


==Structural Information==
*Known structures with covalent ligands: 
:[https://www.rcsb.org/structure/1NFB 1NFB], [https://www.rcsb.org/structure/1B3O 1B3O] 

*Protein structure:
[[File:498.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00230 Purine metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00983 Drug metabolism - other enzymes] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] 

==Experimental Evidence==
:Crystallography, MALDI-TOF/MS, Tryptic Digest, Bio-Rad Protein Assay

==Reference==
# Wang W, Papov V V, Minakawa N, et al. '''Inactivation of inosine 5 ‘-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-β-d-ribofuranosylimidazole-4-carboxamide 5 ‘-monophosphate[J].''' Biochemistry, 1996, 35(1): 95-101. [https://www.ncbi.nlm.nih.gov/pubmed/?term=8555204 8555204] 
# Colby T D, Vanderveen K, Strickler M D, et al. '''Crystal structure of human type II inosine monophosphate dehydrogenase: implications for ligand binding and drug design[J].''' Proceedings of the National Academy of Sciences, 1999, 96(7): 3531-3536. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10097070 10097070] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:IMPDH/GMPR family|IMPDH/GMPR family]]
[[Category:Purine metabolism|Purine metabolism]]
[[Category:Drug metabolism - other enzymes|Drug metabolism - other enzymes]]
[[Category:Metabolic pathways|Metabolic pathways]]

@@ Line 50: / Line 50: @@
 # Colby T D, Vanderveen K, Strickler M D, et al. '''Crystal structure of human type II inosine monophosphate dehydrogenase: implications for ligand binding and drug design[J].''' Proceedings of the National Academy of Sciences, 1999, 96(7): 3531-3536. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10097070 10097070]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Metabolic enzyme]]
-[[Category:IMPDH/GMPR family|IMPDH/GMPR family]]
+[[Category:IMPDH/GMPR family]]
-[[Category:Purine metabolism|Purine metabolism]]
+[[Category:Purine metabolism]]
-[[Category:Drug metabolism - other enzymes|Drug metabolism - other enzymes]]
+[[Category:Drug metabolism - other enzymes]]
-[[Category:Metabolic pathways|Metabolic pathways]]
+[[Category:Metabolic pathways]]

@@ Line 47: / Line 47: @@
 ==Reference==
-# Wang W, Papov V V, Minakawa N, et al. '''Inactivation of inosine 5 ‘-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-β-d-ribofuranosylimidazole-4-carboxamide 5 ‘-monophosphate[J].''' Biochemistry, 1996, 35(1): 95-101. [https://www.ncbi.nlm.nih.gov/pubmed/?term=8555204 8555204]<br/>
+# Wang W, Papov V V, Minakawa N, et al. '''Inactivation of inosine 5'-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-β-d-ribofuranosylimidazole-4-carboxamide 5'-monophosphate[J].''' Biochemistry, 1996, 35(1): 95-101. [https://www.ncbi.nlm.nih.gov/pubmed/?term=8555204 8555204]<br/>
 # Colby T D, Vanderveen K, Strickler M D, et al. '''Crystal structure of human type II inosine monophosphate dehydrogenase: implications for ligand binding and drug design[J].''' Proceedings of the National Academy of Sciences, 1999, 96(7): 3531-3536. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10097070 10097070]<br/>

Inosine-5'-monophosphate dehydrogenase 2 - Revision history

Wu3857: /* Reference */

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|IMPDH2|[https://www.uniprot.org/uniprot/P12268 P12268]|Homo sapiens|Cys331|[http://pfam.xfam.org/family/PF00478..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|IMPDH2|[https://www.uniprot.org/uniprot/P12268 P12268]|Homo sapiens|Cys331|[http://pfam.xfam.org/family/PF00478..."