Wu3857: /* Reference */

2019-08-19T23:00:46Z

‎Reference

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|KAT2B, PCAF|[https://www.uniprot.org/uniprot/Q92831 Q92831]|Homo sapiens|Cys574|[http://pfam.xfam.org/family/PF..."

2019-08-15T03:02:53Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|KAT2B, PCAF|[https://www.uniprot.org/uniprot/Q92831 Q92831]|Homo sapiens|Cys574|[http://pfam.xfam.org/family/PF..."

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{{#invoke:InfoboxforTarget|run|KAT2B, PCAF|[https://www.uniprot.org/uniprot/Q92831 Q92831]|Homo sapiens|Cys574|[http://pfam.xfam.org/family/PF00583 Acetyltransferase family], GCN5 subfamily|[[:Category:Histone acetyltransferase KAT2B|Ligand list]]|Transcription Related, Post-translational Modification}}

==Summary==

===Protein Function ===
K(lysine) acetyltransferase 2B (KAT2B), also known as P300/CBP-associated factor (PCAF), is a human gene and transcriptional coactivator associated with p53. 
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. (From Wikipedia) 

===Cys Function & Property===
Cys574 is close to the active site Glu570 of KAT2B, which act as the nucleophile group in catalyze. 

* Hydrophobic property:
:[[File:560-hydro.png||600px]]
* SASA：
:Cys274: 7.407 A^2 

==Protein Sequence==

MSEAGGAGPG GCGAGAGAGA GPGALPPQPA ALPPAPPQGS PCAAAAGGSG 
ACGPATAVAA AGTAEGPGGG GSARIAVKKA QLRSAPRAKK LEKLGVYSAC 
KAEESCKCNG WKNPNPSPTP PRADLQQIIV SLTESCRSCS HALAAHVSHL 
ENVSEEEMNR LLGIVLDVEY LFTCVHKEED ADTKQVYFYL FKLLRKSILQ 
RGKPVVEGSL EKKPPFEKPS IEQGVNNFVQ YKFSHLPAKE RQTIVELAKM 
FLNRINYWHL EAPSQRRLRS PNDDISGYKE NYTRWLCYCN VPQFCDSLPR 
YETTQVFGRT LLRSVFTVMR RQLLEQARQE KDKLPLEKRT LILTHFPKFL 
SMLEEEVYSQ NSPIWDQDFL SASSRTSQLG IQTVINPPPV AGTISYNSTS 
SSLEQPNAGS SSPACKASSG LEANPGEKRK MTDSHVLEEA KKPRVMGDIP 
MELINEVMST ITDPAAMLGP ETNFLSAko RDEAARLEER RGVIEFHVVG 
NSLNQKPNKK ILMWLVGLQN VFSHQLPRMP KEYITRLVFD PKHKTLALIK 
DGRVIGGICF RMFPSQGFTE IVF'''C'''AVTSNE QVKGYGTHLM NHLKEYHIKH 
DILNFLTYAD EYAIGYFKKQ GFSKEIKIPK TKYVGYIKDY EGATLMGCEL 
NPRIPYTEFS VIIKKQKEII KKLIERKQAQ IRKVYPGLSC FKDGVRQIPI 
ESIPGIRETG WKPSGKEKSK EPRDPDQLYS TLKSILQQVK SHQSAWPFME 
PVKRTEAPGY YEVIRFPMDL KTMSERLKNR YYVSKKLFMA DLQRVFTNCK 
EYNPPESEYY KCANILEKFF FSKIKEAGLI DK 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:[[File:560.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04330 Notch signaling pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04919 Thyroid hormone signaling pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05166 Human T-cell leukemia virus 1 infection] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05203 Viral carcinogenesis] 

==Experimental Evidence==
:LC/MS, Homologous Analysis of Sequence, Enzymatic Assay, Molecular Docking

==Reference==
# Stimson L, Rowlands M G, Newbatt Y M, et al. '''Isothiazolones as inhibitors of PCAF and p300 histone acetyltransferase activity[J].''' Molecular cancer therapeutics, 2005, 4(10): 1521-1532. [https://www.ncbi.nlm.nih.gov/pubmed/?term=16227401 16227401] 
# Wisastra R, Ghizzoni M, Maarsingh H, et al. '''Isothiazolones; thiol-reactive inhibitors of cysteine protease cathepsin B and histone acetyltransferase PCAF[J].''' Organic & biomolecular chemistry, 2011, 9(6): 1817-1822. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21267493 21267493] 
# Dekker F J, Ghizzoni M, van der Meer N, et al. '''Inhibition of the PCAF histone acetyl transferase and cell proliferation by isothiazolones[J].''' Bioorganic & medicinal chemistry, 2009, 17(2): 460-466. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19111471 19111471] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Post-translational Modification|Post-translational Modification]]
[[Category:Transcription Related|Transcription Related]]
[[Category:Acetyltransferase family|Acetyltransferase family]]
[[Category:GCN5 subfamily|GCN5 subfamily]]
[[Category:Notch signaling pathway|Notch signaling pathway]]
[[Category:Thyroid hormone signaling pathway|Thyroid hormone signaling pathway]]
[[Category:Human T-cell leukemia virus 1 infection|Human T-cell leukemia virus 1 infection]]
[[Category:Viral carcinogenesis|Viral carcinogenesis]]

@@ Line 60: / Line 60: @@
 # Dekker F J, Ghizzoni M, van der Meer N, et al. '''Inhibition of the PCAF histone acetyl transferase and cell proliferation by isothiazolones[J].''' Bioorganic & medicinal chemistry, 2009, 17(2): 460-466. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19111471 19111471]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:Transcription Related|Transcription Related]]
+[[Category:Transcription Related]]
-[[Category:Acetyltransferase family|Acetyltransferase family]]
+[[Category:Acetyltransferase family]]
-[[Category:GCN5 subfamily|GCN5 subfamily]]
+[[Category:GCN5 subfamily]]
-[[Category:Notch signaling pathway|Notch signaling pathway]]
+[[Category:Notch signaling pathway]]
-[[Category:Thyroid hormone signaling pathway|Thyroid hormone signaling pathway]]
+[[Category:Thyroid hormone signaling pathway]]
-[[Category:Human T-cell leukemia virus 1 infection|Human T-cell leukemia virus 1 infection]]
+[[Category:Human T-cell leukemia virus 1 infection]]
-[[Category:Viral carcinogenesis|Viral carcinogenesis]]
+[[Category:Viral carcinogenesis]]

Histone acetyltransferase KAT2B - Revision history

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|KAT2B, PCAF|[https://www.uniprot.org/uniprot/Q92831 Q92831]|Homo sapiens|Cys574|[http://pfam.xfam.org/family/PF..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|KAT2B, PCAF|[https://www.uniprot.org/uniprot/Q92831 Q92831]|Homo sapiens|Cys574|[http://pfam.xfam.org/family/PF..."