Glutathione S-transferase P - Revision history

Wu3857: /* Protein Function */

2020-01-22T05:55:06Z

‎Protein Function

Wu3857: /* Protein Function */

2020-01-22T05:54:41Z

‎Protein Function

Wu3857: /* Reference */

2019-08-19T22:52:46Z

‎Reference

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|GSTP1-1, GST class-pi|[https://www.uniprot.org/uniprot/P09211 P09211]|Homo sapiens|Cys15, Cys48, Cys102|[http:/..."

2019-07-30T19:31:37Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|GSTP1-1, GST class-pi|[https://www.uniprot.org/uniprot/P09211 P09211]|Homo sapiens|Cys15, Cys48, Cys102|[http:/..."

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{| align="left"
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{{#invoke:InfoboxforTarget|run|GSTP1-1, GST class-pi|[https://www.uniprot.org/uniprot/P09211 P09211]|Homo sapiens|Cys15, Cys48, Cys102|[http://pfam.xfam.org/family/PF02798 Glutathione S-transferase, N-terminal domain], [http://pfam.xfam.org/family/PF14497 Glutathione S-transferase, C-terminal domain], GST superfamily, Pi family|[[:Category:Glutathione S-transferase P|Ligand list]]|Metabolic enzyme}}
==Summary==

===Protein Function ===
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. (From Uniprot) 
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. 
The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. (From Wikipedia)
 

===Cys Function & Property===
Cys15 and Cys48 are very close to the binding site of Glutathione. 

* Hydrophobic property:
:[[File:501-hydro.png||600px]]
* SASA：
:Cys15: 0 A^2
:Cys48: 5.892 A^2
:Cys102: 50.785 A^2

==Protein Sequence==

MPPYTVVYFP VRGR'''C'''AALRM LLADQGQSWK EEVVTVETWQ EGSLKAS'''C'''LY 
GQLPKFQDGD LTLYQSNTIL RHLGRTLGLY GKDQQEAALV DMVNDGVEDL 
R'''C'''KYISLIYT NYEAGKDDYV KALPGQLKPF ETLLSQNQGG KTFIVGDQIS 
FADYNLLDLL LIHEVLAPGC LDAFPLLSAY VGRLSARPKL KAFLASPEYV 
NLPINGNGKQ 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:501.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00480 Glutathione metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00980 Metabolism of xenobiotics by cytochrome P450] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00982 Drug metabolism - cytochrome P450] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00983 Drug metabolism - other enzymes] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01524 Platinum drug resistance] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05200 Pathways in cancer] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05204 Chemical carcinogenesis] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05215 Prostate cancer] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05225 Hepatocellular carcinoma] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05418 Fluid shear stress and atherosclerosis] 

==Experimental Evidence==
:Cys Modification Assay, LC-MS/MS, Tryptic Digest, Back-titration Experiment, Isothermal Titration Calorimetry, Cys-directed Mutation

==Reference==
# van Iersel M L P S, Ploemen J P H T M, Bello M L, et al. '''Interactions of α, β-unsaturated aldehydes and ketones with human glutathione S-transferase P1-1[J].''' Chemico-biological interactions, 1997, 108(1-2): 67-78. [https://www.ncbi.nlm.nih.gov/pubmed/?term=9463521 9463521] 
# Boerma J S, Dragovic S, Vermeulen N P E, et al. '''Mass spectrometric characterization of protein adducts of multiple P450-dependent reactive intermediates of diclofenac to human glutathione-S-transferase P1-1[J].''' Chemical research in toxicology, 2012, 25(11): 2532-2541. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22998212 22998212] 
# Meng X, Howarth A, Earnshaw C J, et al. '''Detection of drug bioactivation in vivo: mechanism of nevirapine–albumin conjugate formation in patients[J].''' Chemical research in toxicology, 2013, 26(4): 575-583. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23448204 23448204] 
# Quesada‐Soriano I, Parker L J, Primavera A, et al. '''Diuretic drug binding to human glutathione transferase P1‐1: potential role of Cys‐101 revealed in the double mutant C47S/Y108V[J].''' Journal of Molecular Recognition, 2011, 24(2): 220-234. [https://www.ncbi.nlm.nih.gov/pubmed/?term=20540076 20540076] 
# Callan H E, Jenkins R E, Maggs J L, et al. '''Multiple adduction reactions of nitroso sulfamethoxazole with cysteinyl residues of peptides and proteins: implications for hapten formation[J].''' Chemical research in toxicology, 2009, 22(5): 937-948. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19358516 19358516] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:GST superfamily|GST superfamily]]
[[Category:Pi family|Pi family]]
[[Category:Glutathione metabolism|Glutathione metabolism]]
[[Category:Metabolism of xenobiotics by cytochrome P450|Metabolism of xenobiotics by cytochrome P450]]
[[Category:Drug metabolism - cytochrome P450|Drug metabolism - cytochrome P450]]
[[Category:Drug metabolism - other enzymes|Drug metabolism - other enzymes]]
[[Category:Metabolic pathways|Metabolic pathways]]
[[Category:Platinum drug resistance|Platinum drug resistance]]
[[Category:Pathways in cancer|Pathways in cancer]]
[[Category:Chemical carcinogenesis|Chemical carcinogenesis]]
[[Category:Prostate cancer|Prostate cancer]]
[[Category:Hepatocellular carcinoma|Hepatocellular carcinoma]
[[Category:Fluid shear stress and atherosclerosis|Fluid shear stress and atherosclerosis]]

@@ Line 8: / Line 8: @@
 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. (From Uniprot)<br/>
-Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.<br/>
+Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into four main classes: alpha, mu, pi, and theta.<br/>
 The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. (From Wikipedia)

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. (From Uniprot)<br/>
 Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.<br/>
 The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. (From Wikipedia)
 <br/>

@@ Line 60: / Line 60: @@
 # Callan H E, Jenkins R E, Maggs J L, et al. '''Multiple adduction reactions of nitroso sulfamethoxazole with cysteinyl residues of peptides and proteins: implications for hapten formation[J].''' Chemical research in toxicology, 2009, 22(5): 937-948. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19358516 19358516]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Metabolic enzyme]]
-[[Category:GST superfamily|GST superfamily]]
+[[Category:GST superfamily]]
-[[Category:Pi family|Pi family]]
+[[Category:Pi family]]
-[[Category:Glutathione metabolism|Glutathione metabolism]]
+[[Category:Glutathione metabolism]]
-[[Category:Metabolism of xenobiotics by cytochrome P450|Metabolism of xenobiotics by cytochrome P450]]
+[[Category:Metabolism of xenobiotics by cytochrome P450]]
-[[Category:Drug metabolism - cytochrome P450|Drug metabolism - cytochrome P450]]
+[[Category:Drug metabolism - cytochrome P450]]
-[[Category:Drug metabolism - other enzymes|Drug metabolism - other enzymes]]
+[[Category:Drug metabolism - other enzymes]]
-[[Category:Metabolic pathways|Metabolic pathways]]
+[[Category:Metabolic pathways]]
-[[Category:Platinum drug resistance|Platinum drug resistance]]
+[[Category:Platinum drug resistance]]
-[[Category:Pathways in cancer|Pathways in cancer]]
+[[Category:Pathways in cancer]]
-[[Category:Chemical carcinogenesis|Chemical carcinogenesis]]
+[[Category:Chemical carcinogenesis]]
-[[Category:Prostate cancer|Prostate cancer]]
+[[Category:Prostate cancer]]
-[[Category:Hepatocellular carcinoma|Hepatocellular carcinoma]
+[[Category:Hepatocellular carcinoma]]
-[[Category:Fluid shear stress and atherosclerosis|Fluid shear stress and atherosclerosis]]
+[[Category:Fluid shear stress and atherosclerosis]]

Glutathione S-transferase P - Revision history

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|GSTP1-1, GST class-pi|[https://www.uniprot.org/uniprot/P09211 P09211]|Homo sapiens|Cys15, Cys48, Cys102|[http:/..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|GSTP1-1, GST class-pi|[https://www.uniprot.org/uniprot/P09211 P09211]|Homo sapiens|Cys15, Cys48, Cys102|[http:/..."