Wu3857: /* Related Pathway */

2019-12-02T05:10:14Z

‎Related Pathway

Wu3857: /* Cys Function & Property */

2019-12-02T05:09:54Z

‎Cys Function & Property

Wu3857: /* Cys Function & Property */

2019-12-02T05:09:44Z

‎Cys Function & Property

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|FAAH, FAAH1|[https://www.uniprot.org/uniprot/P97612 P97612]|Rattus norvegicus|Cys269|[http://pfam.xfam.org/fami..."

2019-12-02T05:09:25Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|FAAH, FAAH1|[https://www.uniprot.org/uniprot/P97612 P97612]|Rattus norvegicus|Cys269|[http://pfam.xfam.org/fami..."

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{{#invoke:InfoboxforTarget|run|FAAH, FAAH1|[https://www.uniprot.org/uniprot/P97612 P97612]|Rattus norvegicus|Cys269|[http://pfam.xfam.org/family/PF01425 Amidase family]|[[:Category:Fatty-acid amide hydrolase 1|Ligand list]]|Metabolic enzyme}}
==Summary==

===Protein Function ===
Fatty acid amide hydrolase or FAAH (EC 3.5.1.99, oleamide hydrolase, anandamide amidohydrolase) is a member of the serine hydrolase family of enzymes. FAAH is an integral membrane hydrolase with a single N-terminal transmembrane domain. In vitro, FAAH has esterase and amidase activity. In vivo, FAAH is the principal catabolic enzyme for a class of bioactive lipids called the fatty acid amides (FAAs). (From Wikipedia) 
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. (From Uniprot)
 

===Cys Function & Property===
Cys269 is close to the active sites of FAAH.</div>

* Hydrophobic property:
:[[File:589-hydro.png||600px]]
* SASA:
:Cys269: 13.878 A^2 

==Protein Sequence==

MVLSEVWTTL SGVSGVCLAC SLLSAAVVLR WTGRQKARGA ATRARQKQRA 
SLETMDKAVQ RFRLQNPDLD SEALLTLPLL QLVQKLQSGE LSPEAVFFTY 
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH 
DSTLGLSLNE GMPSESDCVV VQVLKLQGAV PFVHTNVPQS MLSFDCSNPL 
FGQTMNPWKS SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG 
ICGLKPTGNR LSKSGLKG'''C'''V YGQTAVQLSL GPMARDVESL ALCLKALLCE 
HLFTLDPTVP PLPFREEVYR SSRPLRVGYY ETDNYTMPSP AMRRALIETK 
QRLEAAGHTL IPFLPNNIPY ALEVLSAGGL FSDGGRSFLQ NFKGDFVDPC 
LGDLILILRL PSWFKRLLSL LLKPLFPRLA AFLNSMRPRS AEKLWKLQHE 
IEMYRQSVIA QWKAMNLDVL LTPMLGPALD LNTPGRATGA ISYTVLYNCL 
DFPAGVVPVT TVTAEDDAQM ELYKGYFGDI WDIILKKAMK NSVGLPVAVQ 
CVALPWQEEL CLRFMREVEQ LMTPQKQPS 


==Structural Information==
*Known structure with covalent ligand: 
:[https://www.rcsb.org/structure/4J5P 4J5P] 

*Protein structure:
[[File:589.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?>ko04723 Retrograde endocannabinoid signaling] <br/

==Experimental Evidence==
:Cys-directed mutation, Homologous Analysis Of Sequence, Molecular Docking

==Reference==
# Otrubova K, Cravatt B F, Boger D L. '''Design, synthesis, and characterization of α-ketoheterocycles that additionally target the cytosolic port Cys269 of fatty acid amide hydrolase[J].''' Journal of medicinal chemistry, 2014, 57(3): 1079-1089. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24456116 24456116] 
# Otrubova K, Brown M, McCormick M S, et al. '''Rational design of fatty acid amide hydrolase inhibitors that act by covalently bonding to two active site residues[J].''' Journal of the American Chemical Society, 2013, 135(16): 6289-6299. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23581831 23581831] 

[[Category:Targets]]
[[Category:Rattus norvegicus]]
[[Category:Metabolic enzyme]]
[[Category:Amidase family]]
[[Category:Retrograde endocannabinoid signaling]]

@@ Line 11: / Line 11: @@
 ===Cys Function & Property===
-Cys269 is close to the active sites of FAAH.<br/>
+Cys269 is close to the active sites of FAAH in space.<br/>
 * Hydrophobic property:

@@ Line 42: / Line 42: @@
 ==Related Pathway==
-*[https://www.genome.jp/kegg-bin/show_pathway?>ko04723 Retrograde endocannabinoid signaling] <br/
+*[https://www.genome.jp/kegg-bin/show_pathway?ko04723 Retrograde endocannabinoid signaling] <br/>
 ==Experimental Evidence==

Fatty-acid amide hydrolase 1 - Revision history

Wu3857: /* Related Pathway */

Wu3857: /* Cys Function & Property */

Wu3857: /* Cys Function & Property */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|FAAH, FAAH1|[https://www.uniprot.org/uniprot/P97612 P97612]|Rattus norvegicus|Cys269|[http://pfam.xfam.org/fami..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|FAAH, FAAH1|[https://www.uniprot.org/uniprot/P97612 P97612]|Rattus norvegicus|Cys269|[http://pfam.xfam.org/fami..."