Dual specificity phosphatase Cdc25B - Revision history

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2019-08-19T22:17:43Z

‎Reference

Wu3857 at 20:36, 7 August 2019

2019-08-07T20:36:35Z

Wu3857 at 15:11, 1 August 2019

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Wu3857 at 15:01, 1 August 2019

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Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CDC25B, M-phase inducer phosphatase 2|[https://www.uniprot.org/uniprot/P30305 P30305]|Homo sapiens|Cys440, Cys4..."

2019-08-01T15:00:50Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CDC25B, M-phase inducer phosphatase 2|[https://www.uniprot.org/uniprot/P30305 P30305]|Homo sapiens|Cys440, Cys4..."

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{{#invoke:InfoboxforTarget|run|CDC25B, M-phase inducer phosphatase 2|[https://www.uniprot.org/uniprot/P30305 P30305]|Homo sapiens|Cys440, Cys486|[http://pfam.xfam.org/family/PF06617 M-phase inducer phosphatase (MPI) phosphatase family]|[[:Category:Dual specificity phosphatase Cdc25B|Ligand list]]|Transcription factor}}
==Summary==

===Protein Function ===
M-phase inducer phosphatase 2 is an enzyme that in humans is encoded by the CDC25B gene. 
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. (From Wikipedia)
 

===Cys Function & Property===
Cys486 is the active site of CDC25B, and Cys440 is very close to this site in space. 

* Hydrophobic property:
:[[File:517-hydro.png||600px]]
* SASA：
:Cys440: 0.411 A^2 
:Cys486: 2.207 A^2 

==Protein Sequence==

MEVPQPEPAP GSALSPAGVC GGAQRPGHLP GLLLGSHGLL GSPVRAAASS 
PVTTLTQTMH DLAGLGSETP KSQVGTLLFR SRSRLTHLSL SRRASESSLS 
SESSESSDAG LCMDSPSPMD PHMAEQTFEQ AIQAASRIIR NEQFAIRRFQ 
SMPVRLLGHS PVLRNITNSQ APDGRRKSEA GSGAASSSGE DKENDGFVFK 
MPWKPTHPSS THALAEWASR REAFAQRPSS APDLMCLSPD RKMEVEELSP 
LALGRFSLTP AEGDTEEDDG FVDILESDLK DDDAVPPGME SLISAPLVKT 
LEKEEEKDLV MYSKCQRLFR SPSMPCSVIR PILKRLERPQ DRDTPVQNKR 
RRSVTPPEEQ QEAEEPKARV LRSKSLCHDE IENLLDSDHR ELIGDYSKAF 
LLQTVDGKHQ DLKYISPETM VALLTGKFSN IVDKFVIVD'''C''' RYPYEYEGGH 
IKTAVNLPLE RDAESFLLKS PIAPCSLDKR VILIFH'''C'''EFS SERGPRMCRF 
IRERDRAVND YPSLYYPEMY ILKGGYKEFF PQHPNFCEPQ DYRPMNHEAF 
KDELKTFRLK TRSWAGERSR RELCSRLQDQ 


==Structural Information==
*Known structure with covalent ligand: 
:[https://www.rcsb.org/structure/1CWS 1CWS], [https://www.rcsb.org/structure/1CWT 1CWT] 

*Protein structure:
::[[File:517-2.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04010 MAPK signaling pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04110 Cell cycle] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04914 Progesterone-mediated oocyte maturation] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05206 MicroRNAs in cancer] 

==Experimental Evidence==
:Crystallography, MALDI-TOF/MS, Western Blot, Endoproteinase Lys-C Digest, Tryptic Digest

==Reference==
# Reynolds R A, Yem A W, Wolfe C L, et al. '''Crystal structure of the catalytic subunit of Cdc25B required for G2/M phase transition of the cell cycle[J].''' Journal of molecular biology, 1999, 293(3): 559-568. [https://www.ncbi.nlm.nih.gov/pubmed/?term=10543950 10543950] 
# Tsuchiya A, Asanuma M, Hirai G, et al. '''CDC25A-inhibitory RE derivatives bind to pocket adjacent to the catalytic site[J].''' Molecular BioSystems, 2013, 9(5): 1026-1034. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23467652 23467652] 
# Ueda K, Usui T, Nakayama H, et al. '''4‐Isoavenaciolide covalently binds and inhibits VHR, a dual‐specificity phosphatase[J].''' FEBS letters, 2002, 525(1-3): 48-52. [https://www.ncbi.nlm.nih.gov/pubmed/?term=12163160 12163160] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Phosphatase|Phosphatase]]
[[Category:MPI phosphatase family|MPI phosphatase family]]
[[Category:MAPK signaling pathway|MAPK signaling pathway]]
[[Category:Cell cycle|Cell cycle]]
[[Category:Progesterone-mediated oocyte maturation|Progesterone-mediated oocyte maturation]]
[[Category:MicroRNAs in cancer|MicroRNAs in cancer]]

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 # Ueda K, Usui T, Nakayama H, et al. '''4‐Isoavenaciolide covalently binds and inhibits VHR, a dual‐specificity phosphatase[J].''' FEBS letters, 2002, 525(1-3): 48-52. [https://www.ncbi.nlm.nih.gov/pubmed/?term=12163160 12163160]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Phosphatase|Phosphatase]]
+[[Category:Phosphatase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:MPI phosphatase family|MPI phosphatase family]]
+[[Category:MPI phosphatase family]]
-[[Category:MAPK signaling pathway|MAPK signaling pathway]]
+[[Category:MAPK signaling pathway]]
-[[Category:Cell cycle|Cell cycle]]
+[[Category:Cell cycle]]
-[[Category:Progesterone-mediated oocyte maturation|Progesterone-mediated oocyte maturation]]
+[[Category:Progesterone-mediated oocyte maturation]]
-[[Category:MicroRNAs in cancer|MicroRNAs in cancer]]
+[[Category:MicroRNAs in cancer]]