Dual specificity phosphatase Cdc25A - Revision history

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2019-08-19T22:17:06Z

‎Reference

Wu3857 at 20:36, 7 August 2019

2019-08-07T20:36:08Z

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2019-08-07T20:35:35Z

Wu3857 at 15:17, 1 August 2019

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Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CDC25A, M-phase inducer phosphatase 1|[https://www.uniprot.org/uniprot/P30304 P30304]|Homo sapiens|Cys385|[http..."

2019-08-01T15:16:56Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CDC25A, M-phase inducer phosphatase 1|[https://www.uniprot.org/uniprot/P30304 P30304]|Homo sapiens|Cys385|[http..."

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{{#invoke:InfoboxforTarget|run|CDC25A, M-phase inducer phosphatase 1|[https://www.uniprot.org/uniprot/P30304 P30304]|Homo sapiens|Cys385|[http://pfam.xfam.org/family/PF06617 M-phase inducer phosphatase (MPI) phosphatase family]|[[:Category:Dual specificity phosphatase Cdc25A|Ligand list]]|Phosphatase}}
==Summary==

===Protein Function ===
M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A is a protein that in humans is encoded by the cell division cycle 25 homolog A (CDC25A) gene. CDC25A is a member of the CDC25 family of dual-specificity phosphatases. Dual-specificity protein phosphatases remove phosphate groups from phosphorylated tyrosine and serine/threonine residues. They represent a subgroup of the tyrosine phosphatase family (as opposed to the serine/threonine phosphatase family). All mammals examined to date have three homologues of the ancestral Cdc25 gene (found e.g. in the fungus species S. pombe , designated Cdc25A, Cdc25B, and Cdc25C. In contrast, some invertebrates harbour 2 (e.g., the Drosophila proteins String and Twine) or four (e.g., C. elegans Cdc-25.1 - Cdc-25.4) homologues. CDC25A is required for progression from G1 to the S phase of the cell cycle, but also plays roles in later cell cycle events. In particular, it is stabilized in metaphase cells and is degraded upon metaphase exit akin to Cyclin B. It is competent to activate the G1/S cyclin-dependent kinases CDK4 and CDK2 by removing inhibitory phosphate groups from adjacent tyrosine and threonine residues; it can also activate Cdc2 (Cdk1), the principal mitotic Cdk. 
Cell division cycle 25 molecules (CDC25s) are members of the dual-specificity protein phosphatase (DSP) family of enzymes that dephosphorylate both phospho-serine/threonine and phosphotyrosine residues in the same protein. CDC25s dephosphorylate conserved phospho-tyrosine and phospho-threonine residues on cyclin-dependent kinases (CDKs), thereby activating the kinases and promoting cell cycle progression and transition. In addition to these functions, CDC25s play an important role as checkpoint regulators for handling DNA damage caused by UV light, ionizing irradiation, or chemicals. Therefore, genomic instability is likely to be triggered by misregulation of CDC25s. Mammalian cells express three CDC25 isoforms, CDC25A, CDC25B, and CDC25C. (PMID: 23467652)
CDC25A is required for progression from G1 to the S phase of the cell cycle, but also plays roles in later cell cycle events. In particular, it is stabilized in metaphase cells and is degraded upon metaphase exit akin to Cyclin B. It is competent to activate the G1/S cyclin-dependent kinases CDK4 and CDK2 by removing inhibitory phosphate groups from adjacent tyrosine and threonine residues; it can also activate Cdc2 (Cdk1), the principal mitotic Cdk. (From Wikipedia)
 

===Cys Function & Property===
Cys431 is the active site of CDC25B, and Cys385 is very close to this site in space. 

* Hydrophobic property:
:[[File:516-hydro.png||600px]]
* SASA：
:Cys385: 0 A^2 

==Protein Sequence==

MELGPEPPHR RRLLFACSPP PASQPVVKAL FGASAAGGLS PVTNLTVTMD 
QLQGLGSDYE QPLEVKNNSN LQRMGSSEST DSGFCLDSPG PLDSKENLEN 
PMRRIHSLPQ KLLGCSPALK RSHSDSLDHD IFQLIDPDEN KENEAFEFKK 
PVRPVSRGCL HSHGLQEGKD LFTQRQNSAP ARMLSSNERD SSEPGNFIPL 
FTPQSPVTAT LSDEDDGFVD LLDGENLKNE EETPSCMASL WTAPLVMRTT 
NLDNRCKLFD SPSLCSSSTR SVLKRPERSQ EESPPGSTKR RKSMSGASPK 
ESTNPEKAHE TLHQSLSLAS SPKGTIENIL DNDPRDLIGD FSKGYLFHTV 
AGKHQDLKYI SPEIMASVLN GKFANLIKEF VIID'''C'''RYPYE YEGGHIKGAV 
NLHMEEEVED FLLKKPIVPT DGKRVIVVFH CEFSSERGPR MCRYVRERDR 
LGNEYPKLHY PELYVLKGGY KEFFMKCQSY CEPPSYRPMH HEDFKEDLKK 
FRTKSRTWAG EKSKREMYSR LKKL 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
::[[File:516.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04218 Cellular senescence] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04110 Cell cycle] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04914 Progesterone-mediated oocyte maturation] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05206 MicroRNAs in cancer] 

==Experimental Evidence==
:MALDI-TOF/MS, Western Blot, Endoproteinase Lys-C Digest, Tryptic Digest

==Reference==
# Tsuchiya A, Asanuma M, Hirai G, et al. '''CDC25A-inhibitory RE derivatives bind to pocket adjacent to the catalytic site[J].''' Molecular BioSystems, 2013, 9(5): 1026-1034. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23467652 23467652] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Phosphatase|Phosphatase]]
[[Category:MPI phosphatase family|MPI phosphatase family]]
[[Category:Cellular senescence|Cellular senescence]]
[[Category:Cell cycle|Cell cycle]]
[[Category:Progesterone-mediated oocyte maturation|Progesterone-mediated oocyte maturation]]
[[Category:MicroRNAs in cancer|MicroRNAs in cancer]]

@@ Line 53: / Line 53: @@
 # Tsuchiya A, Asanuma M, Hirai G, et al. '''CDC25A-inhibitory RE derivatives bind to pocket adjacent to the catalytic site[J].''' Molecular BioSystems, 2013, 9(5): 1026-1034. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23467652 23467652]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Phosphatase|Phosphatase]]
+[[Category:Phosphatase]]
-[[Category:Post-translational Modification|Post-translational Modification]]
+[[Category:Post-translational Modification]]
-[[Category:MPI phosphatase family|MPI phosphatase family]]
+[[Category:MPI phosphatase family]]
-[[Category:Cellular senescence|Cellular senescence]]
+[[Category:Cellular senescence]]
-[[Category:Cell cycle|Cell cycle]]
+[[Category:Cell cycle]]
-[[Category:Progesterone-mediated oocyte maturation|Progesterone-mediated oocyte maturation]]
+[[Category:Progesterone-mediated oocyte maturation]]
-[[Category:MicroRNAs in cancer|MicroRNAs in cancer]]
+[[Category:MicroRNAs in cancer]]