Wu3857: /* Reference */

2019-08-19T22:16:28Z

‎Reference

Wu3857: /* Cys Function & Property */

2019-08-18T01:29:11Z

‎Cys Function & Property

Wu3857 at 16:19, 17 August 2019

2019-08-17T16:19:53Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300,
Cys392, Cys405|[..."

2019-08-17T16:19:37Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300, Cys392, Cys405|[..."

New page

{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300, Cys392, Cys405|[http://pfam.xfam.org/family/PF00204 DNA gyrase B], Type II topoisomerase family]|[[:Category:DNA topoisomerase 2-alpha|Ligand list]]|Transcription Related}}

==Summary==

===Protein Function ===
TOP2A is an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia (From Wikipedia) 

===Cys Function & Property===
TO be added 

* Hydrophobic property:
:[[File:564-hydro.png||600px]]
* SASA：
:Cys170: 0 A^2 
:Cys216: 15.977 A^2 
:Cys300: 0 A^2 
:Cys392: 0.092 A^2 
:Cys405: 6.811 A^2 

==Protein Sequence==

MEVSPLQPVN ENMQVNKIKK NEDAKKRLSV ERIYQKKTQL EHILLRPDTY 
IGSVELVTQQ MWVYDEDVGI NYREVTFVPG LYKIFDEILV NAADNKQRDP 
KMSCIRVTID PENNLISIWN NGKGIPVVEH KVEKMYVPAL IFGQLLTSSN 
YDDDEKKVTG GRNGYGAKL'''C''' NIFSTKFTVE TASREYKKMF KQTWMDNMGR 
AGEMELKPFN GEDYT'''C'''ITFQ PDLSKFKMQS LDKDIVALMV RRAYDIAGST 
KDVKVFLNGN KLPVKGFRSY VDMYLKDKLD ETGNSLKVIH EQVNHRWEV'''C''' 
LTMSEKGFQQ ISFVNSIATS KGGRHVDYVA DQIVTKLVDV VKKKNKGGVA 
VKAHQVKNHM WIFVNALIEN PTFDSQTKEN MTLQPKSFGS T'''C'''QLSEKFIK 
AAIG'''C'''GIVES ILNWVKFKAQ VQLNKKCSAV KHNRIKGIPK LDDANDAGGR 
NSTECTLILT EGDSAKTLAV SGLGVVGRDK YGVFPLRGKI LNVREASHKQ 
IMENAEINNI IKIVGLQYKK NYEDEDSLKT LRYGKIMIMT DQDQDGSHIK 
GLLINFIHHN WPSLLRHRFL EEFITPIVKV SKNKQEMAFY SLPEFEEWKS 
STPNHKKWKV KYYKGLGTST SKEAKEYFAD MKRHRIQFKY SGPEDDAAIS 
LAFSKKQIDD RKEWLTNFME DRRQRKLLGL PEDYLYGQTT TYLTYNDFIN 
KELILFSNSD NERSIPSMVD GLKPGQRKVL FTCFKRNDKR EVKVAQLAGS 
VAEMSSYHHG EMSLMMTIIN LAQNFVGSNN LNLLQPIGQF GTRLHGGKDS 
ASPRYIFTML SSLARLLFPP KDDHTLKFLY DDNQRVEPEW YIPIIPMVLI 
NGAEGIGTGW SCKIPNFDVR EIVNNIRRLM DGEEPLPMLP SYKNFKGTIE 
ELAPNQYVIS GEVAILNSTT IEISELPVRT WTQTYKEQVL EPMLNGTEKT 
PPLITDYREY HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM 
VLFDHVGCLK KYDTVLDILR DFFELRLKYY GLRKEWLLGM LGAESAKLNN 
QARFILEKID GKIIIENKPK KELIKVLIQR GYDSDPVKAW KEAQQKVPDE 
EENEESDNEK ETEKSDSVTD SGPTFNYLLD MPLWYLTKEK KDELCRLRNE 
KEQELDTLKR KSPSDLWKED LATFIEELEA VEAKEKQDEQ VGLPGKGGKA 
KGKKTQMAEV LPSPRGQRVI PRITIEMKAE AEKKNKKKIK NENTEGSPQE 
DGVELEGLKQ RLEKKQKREP GTKTKKQTTL AFKPIKKGKK RNPWSDSESD 
RSSDESNFDV PPRETEPRRA ATKTKFTMDL DSDEDFSDFD EKTDDEDFVP 
SDASPPKTKT SPKLSNKELK PQKSVVSDLE ADDVKGSVPL SSSPPATHFP 
DETEITNPVP KKNVTVKKTA AKSQSSTSTT GAKKRAAPKG TKRDPALNSG 
VSQKPDPAKT KNRRKRKPST SDDSDSNFEK IVSKAVTSKK SKGESDDFHM 
DFDSAVAPRA KSVRAKKPIK YLEESDEDDL F 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:[[File:564.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko01524 Platinum drug resistance] 

==Experimental Evidence==
:Cys-directed Mutation

==Reference==
# Wu X, Yalowich J C, Hasinoff B B. '''Cadmium is a catalytic inhibitor of DNA topoisomerase II[J].''' Journal of inorganic biochemistry, 2011, 105(6): 833-838. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21497582 21497582] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Transcription Related|Transcription Related]]
[[Category:Type II topoisomerase family|Type II topoisomerase family]]
[[Category:Platinum drug resistance|Platinum drug resistance]]

@@ Line 71: / Line 71: @@
 # Wu X, Yalowich J C, Hasinoff B B. '''Cadmium is a catalytic inhibitor of DNA topoisomerase II[J].''' Journal of inorganic biochemistry, 2011, 105(6): 833-838. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21497582 21497582]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Transcription Related|Transcription Related]]
+[[Category:Transcription Related]]
-[[Category:Type II topoisomerase family|Type II topoisomerase family]]
+[[Category:Type II topoisomerase family]]
-[[Category:Platinum drug resistance|Platinum drug resistance]]
+[[Category:Platinum drug resistance]]

@@ Line 10: / Line 10: @@
 ===Cys Function & Property===
 * Hydrophobic property:
@@ Line 20: / Line 19: @@
 :Cys392: 0.092 A^2 <br/>
 :Cys405: 6.811 A^2 <br/>
 ==Protein Sequence==

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300,<br/>Cys392, Cys405|[http://pfam.xfam.org/family/PF00204 DNA gyrase B],<br/>Type II topoisomerase family]|[[:Category:DNA topoisomerase 2-alpha|Ligand list]]|Transcription Related}}
+{{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300,<br/>Cys392, Cys405|[http://pfam.xfam.org/family/PF00204 DNA gyrase B],<br/>Type II topoisomerase family|[[:Category:DNA topoisomerase 2-alpha|Ligand list]]|Transcription Related}}
 ==Summary==

DNA topoisomerase 2-alpha - Revision history

Wu3857: /* Reference */

Wu3857: /* Cys Function & Property */

Wu3857 at 16:19, 17 August 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300,Cys392, Cys405|[..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|TOP2A|[https://www.uniprot.org/uniprot/P11388 P11388]|Homo sapiens|Cys170, Cys216, Cys300,
Cys392, Cys405|[..."