DNA repair protein RAD51 homolog 1 - Revision history

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2019-08-19T22:07:17Z

‎Reference

Wu3857: /* Reference */

2019-07-31T20:35:51Z

‎Reference

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|hRAD51, RAD51|[https://www.uniprot.org/uniprot/Q06609 Q06609]|Homo sapiens|Cys319|RecA family,
[http://pfam..."

2019-07-31T20:34:32Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|hRAD51, RAD51|[https://www.uniprot.org/uniprot/Q06609 Q06609]|Homo sapiens|Cys319|RecA family, [http://pfam..."

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{{#invoke:InfoboxforTarget|run|hRAD51, RAD51|[https://www.uniprot.org/uniprot/Q06609 Q06609]|Homo sapiens|Cys319|RecA family, [http://pfam.xfam.org/family/PF08423 RAD51 subfamily]|[[:Category:DNA repair protein RAD51 homolog 1|Ligand list]]|DNA repair enzyme}}
==Summary==

===Protein Function ===
RAD51 plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PMID: 28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PMID: 26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PMID: 26253028). (From Uniprot)
 

===Cys Function & Property===
Cys319 is very close to the ATP and metal ion binding site in space. 

* Hydrophobic property:
:[[File:512-hydro.png||600px]]
* SASA：
:Cys319: 105.372 A^2 

==Protein Sequence==

MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE 
AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII 
QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR 
GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ 
TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR 
MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL 
YLRKGRGETR ICKIYDSP'''C'''L PEAEAMFAIN ADGVGDAKD 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
::[[File:512.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko03440 Homologous recombination] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko03460 Fanconi anemia pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05200 Pathways in cancer] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05212 Pancreatic cancer] 

==Experimental Evidence==
:Fluorescence Polarization Assay, Cys-directed Mutation

==Reference==
# Budke B, Kalin J H, Pawlowski M, et al. '''An optimized RAD51 inhibitor that disrupts homologous recombination without requiring Michael acceptor reactivity[J].''' Journal of medicinal chemistry, 2012, 56(1): 254-263. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23231413 23231413] 
# Budke B, Logan H L, Kalin J H, et al. '''RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells[J].''' Nucleic acids research, 2012, 40(15): 7347-7357. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22573178 22573178] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:DNA repair enzyme|DNA repair enzyme]]
[[Category:RAD51 subfamily|RAD51 subfamily]]
[[Category:RecA family|RecA family]]
[[Category:Homologous recombination|Homologous recombination]]
[[Category:Fanconi anemia pathway|Fanconi anemia pathway]]
[[Category:Pathways in cancer|Pathways in cancer]]
[[Category:Pancreatic cancer|Pancreatic cancer]]

@@ Line 48: / Line 48: @@
 # Budke B, Logan H L, Kalin J H, et al. '''RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells[J].''' Nucleic acids research, 2012, 40(15): 7347-7357. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22573178 22573178]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:DNA repair enzyme|DNA repair enzyme]]
+[[Category:DNA repair enzyme]]
-[[Category:RecA family|RecA family]]
+[[Category:RecA family]]
-[[Category:RAD51 subfamily|RAD51 subfamily]]
+[[Category:RAD51 subfamily]]
-[[Category:Homologous recombination|Homologous recombination]]
+[[Category:Homologous recombination]]
-[[Category:Fanconi anemia pathway|Fanconi anemia pathway]]
+[[Category:Fanconi anemia pathway]]
-[[Category:Pathways in cancer|Pathways in cancer]]
+[[Category:Pathways in cancer]]
-[[Category:Pancreatic cancer|Pancreatic cancer]]
+[[Category:Pancreatic cancer]]

DNA repair protein RAD51 homolog 1 - Revision history

Wu3857: /* Reference */

Wu3857: /* Reference */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|hRAD51, RAD51|[https://www.uniprot.org/uniprot/Q06609 Q06609]|Homo sapiens|Cys319|RecA family,[http://pfam..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|hRAD51, RAD51|[https://www.uniprot.org/uniprot/Q06609 Q06609]|Homo sapiens|Cys319|RecA family,
[http://pfam..."