Choline-phosphate cytidylyltransferase A (Homo sapiens) - Revision history

Wu3857: /* Reference */

2019-08-19T21:59:04Z

‎Reference

Wu3857: /* Protein Function */

2019-08-02T02:46:44Z

‎Protein Function

Wu3857: /* Protein Function */

2019-08-02T02:38:55Z

‎Protein Function

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CCT-alpha, CCT-A, PCYT1A|[https://www.uniprot.org/uniprot/P49585 P49585]|Homo sapiens|Cys37|[http://pfam.xfam.o..."

2019-08-02T02:32:11Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CCT-alpha, CCT-A, PCYT1A|[https://www.uniprot.org/uniprot/P49585 P49585]|Homo sapiens|Cys37|[http://pfam.xfam.o..."

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{| align="left"
| __TOC__
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{{#invoke:InfoboxforTarget|run|CCT-alpha, CCT-A, PCYT1A|[https://www.uniprot.org/uniprot/P49585 P49585]|Homo sapiens|Cys37|[http://pfam.xfam.org/family/PF01467 Cytidylyltransferase-like family]|[[:Category:Choline-phosphate cytidylyltransferase A (Homo sapiens)|Ligand list]]|Metabolic enzyme}}
==Summary==

===Protein Function ===
Choline-phosphate cytidylyltransferase (EC 2.7.7.15) is an enzyme that catalyzes the chemical reaction: 
CTP + choline phosphate ↔ diphosphate + CDP-choline 
It is responsible for regulating phosphatidylcholine content in membranes. 
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). This enzyme participates in aminophosphonate metabolism and glycerophospholipid metabolism. (From Wikipedia) 

The main pathway for Phosphatidylcholine (PtdCho) biosynthesis in mammalian cells is the Kennedy or CDP-choline pathway, which is regulated by the activity of CCT. CCT activity is essential for cell survival as inhibition of CCT activity leading to decreased PtdCho synthesis impairs cell proliferation, induces apoptosiss, and reduces pulmonary surfactant biosynthesis. (PMID: 18614529)
 

===Cys Function & Property===
CCT-alpha exists as an inactive soluble form and a membrane-associated active form. Both soluble and membrane-bound forms are present in cells as a noncovalently associated dimer comprised of a 42-kDa monomer. A pair of Cys37 residues, located within the aminoterminal domain, are thought to be within disulfide bond distance in the soluble form of the enzyme, but after binding activatinglipids, these Cys37 residues shift slightly with increased separation in the lipid-bound dimer. (PMID: 18614529) 

* Hydrophobic property:
:[[File:518-hydro.png||600px]]
* SASA：
:Cys37: Unknown A^2 

==Protein Sequence==

MDAQCSAKVN ARKRRKEAPG PNGATEEDGV PSKVQR'''C'''AVG LRQPAPFSDE 
IEVDFSKPYV RVTMEEASRG TPCERPVRVY ADGIFDLFHS GHARALMQAK 
NLFPNTYLIV GVCSDELTHN FKGFTVMNEN ERYDAVQHCR YVDEVVRNAP 
WTLTPEFLAE HRIDFVAHDD IPYSSAGSDD VYKHIKEAGM FAPTQRTEGI 
STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY HLQERVDKVK 
KKVKDVEEKS KEFVQKVEEK SIDLIQKWEE KSREFIGSFL EMFGPEGALK 
HMLKEGKGRM LQAISPKQSP SSSPTRERSP SPSFRWPFSG KTSPPCSPAN 
LSRHKAAAYD ISEDEED 


==Structural Information==
*Known structure with covalent ligand: 
:Unknown 

*Protein structure:
:Unknown 

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00440 Phosphonate and phosphinate metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko00564 Glycerophospholipid metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05231 Choline metabolism in cancer] 

==Experimental Evidence==
:Western Blot, Cys-directed Mutation

==Reference==
# Ryan A J, Chen B B, Vennalaganti P R, et al. '''15-Deoxy-δ12, 14-prostaglandin J2 impairs phosphatidylcholine synthesis and induces nuclear accumulation of thiol-modified cytidylyltransferase[J].''' Journal of Biological Chemistry, 2008, 283(36): 24628-24640. [https://www.ncbi.nlm.nih.gov/pubmed/?term=18614529 18614529] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:Cytidylyltransferase family|Cytidylyltransferase family]]
[[Category:Phosphonate and phosphinate metabolism|Phosphonate and phosphinate metabolism]]
[[Category:Glycerophospholipid metabolism|Glycerophospholipid metabolism]]
[[Category:Metabolic pathways|Metabolic pathways]]
[[Category:Choline metabolism in cancer|Choline metabolism in cancer]]

@@ Line 52: / Line 52: @@
 # Ryan A J, Chen B B, Vennalaganti P R, et al. '''15-Deoxy-δ12, 14-prostaglandin J2 impairs phosphatidylcholine synthesis and induces nuclear accumulation of thiol-modified cytidylyltransferase[J].''' Journal of Biological Chemistry, 2008, 283(36): 24628-24640. [https://www.ncbi.nlm.nih.gov/pubmed/?term=18614529 18614529]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Metabolic enzyme]]
-[[Category:Cytidylyltransferase family|Cytidylyltransferase family]]
+[[Category:Cytidylyltransferase family]]
-[[Category:Phosphonate and phosphinate metabolism|Phosphonate and phosphinate metabolism]]
+[[Category:Phosphonate and phosphinate metabolism]]
-[[Category:Glycerophospholipid metabolism|Glycerophospholipid metabolism]]
+[[Category:Glycerophospholipid metabolism]]
-[[Category:Metabolic pathways|Metabolic pathways]]
+[[Category:Metabolic pathways]]
-[[Category:Choline metabolism in cancer|Choline metabolism in cancer]]
+[[Category:Choline metabolism in cancer]]

@@ Line 10: / Line 10: @@
 It is responsible for regulating phosphatidylcholine content in membranes.<br/>
 This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). This enzyme participates in aminophosphonate metabolism and glycerophospholipid metabolism. (From Wikipedia)<br/>
 The main pathway for Phosphatidylcholine (PtdCho) biosynthesis in mammalian cells is the Kennedy or CDP-choline pathway, which is regulated by the activity of CCT. CCT activity is essential for cell survival as inhibition of CCT activity leading to decreased PtdCho synthesis impairs cell proliferation, induces apoptosiss, and reduces pulmonary surfactant biosynthesis. (PMID: 18614529)
 <br/>

@@ Line 7: / Line 7: @@
 ===Protein Function ===
 Choline-phosphate cytidylyltransferase (EC 2.7.7.15) is an enzyme that catalyzes the chemical reaction:<br/>
-CTP + choline phosphate ↔ diphosphate + CDP-choline<br/>
+CTP + choline phosphate <font size="4">↔</font> diphosphate + CDP-choline<br/>
 It is responsible for regulating phosphatidylcholine content in membranes.<br/>
 This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). This enzyme participates in aminophosphonate metabolism and glycerophospholipid metabolism. (From Wikipedia)<br/>

Choline-phosphate cytidylyltransferase A (Homo sapiens) - Revision history

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857: /* Protein Function */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CCT-alpha, CCT-A, PCYT1A|[https://www.uniprot.org/uniprot/P49585 P49585]|Homo sapiens|Cys37|[http://pfam.xfam.o..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CCT-alpha, CCT-A, PCYT1A|[https://www.uniprot.org/uniprot/P49585 P49585]|Homo sapiens|Cys37|[http://pfam.xfam.o..."