Cathepsin L2 - Revision history

Wu3857: /* Reference */

2019-08-19T21:53:14Z

‎Reference

Wu3857 at 20:36, 29 July 2019

2019-07-29T20:36:11Z

Wu3857: /* Reference */

2019-07-28T20:24:30Z

‎Reference

Wu3857: /* Structural Information */

2019-07-28T18:18:15Z

‎Structural Information

Wu3857 at 17:49, 28 July 2019

2019-07-28T17:49:45Z

Wu3857: /* Structural Information */

2019-07-28T03:33:54Z

‎Structural Information

Wu3857: /* Protein Function */

2019-07-28T03:33:21Z

‎Protein Function

Wu3857: /* Cys Function & Property */

2019-07-28T03:33:01Z

‎Cys Function & Property

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys13 8|[http://pfam.xfam.org/family/PF00112..."

2019-07-28T03:32:47Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys13 8|[http://pfam.xfam.org/family/PF00112..."

New page

{| align="left"
| __TOC__
|}
{{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys13 8|[http://pfam.xfam.org/family/PF00112 Peptidase C1 family]|[[:Category:Cathepsin L2|Ligand list]]}}
==Summary==

===Protein Function ===
Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. (From Wikipedia) Cathepsin V is a lysosomal cysteine protease that is expressed in the thymus, testis and corneal epithelium. Its sequence shows that it is a member of the papain family of cysteine proteases, and several pieces of evidence demonstrate an especially close link between this enzyme and cathepsin L. The sequences of these two enzymes are quite similar, with a sequence identity of 77% for the proenzyme and 80% for the mature enzyme. In addition, the position and chromosomal organization of the genes coding for these two cathepsins are similar. The cathepsin V gene has been mapped to a region near the cathepsin L locus, and both genes consist of eight exons that code for analogous regions of the cathepsins V and L structures. Cathepsin V may be involved in T-cell selection in humans. (PMID: 11027133)
 

===Cys Function & Property===
Cys138 is one of the active sites of Cathepsin L2, which is very close to His277 and Asn301 in space. These three residues formed a typical catalytic triad motif. 

* Hydrophobic property:
:[[File:485-hydro.png||600px]]
* SASA：
:Cys138: 10.412 A^2

==Protein Sequence==

MNLSLVLAAF CLGIASAVPK FDQNLDTKWY QWKATHRRLY GANEEGWRRA 
VWEKNMKMIE LHNGEYSQGK HGFTMAMNAF GDMTNEEFRQ MMGCFRNQKF 
RKGKVFREPL FLDLPKSVDW RKKGYVTPVK NQKQCGS'''C'''WA FSATGALEGQ 
MFRKTGKLVS LSEQNLVDCS RPQGNQGCNG GFMARAFQYV KENGGLDSEE 
SYPYVAVDEI CKYRPENSVA NDTGFTVVAP GKEKALMKAV ATVGPISVAM 
DAGHSSFQFY KSGIYFEPDC SSKNLDHGVL VVGYGFEGAN SNNSKYWLVK 
NSWGPEWGSN GYVKIAKDKN NHCGIATAAS YPNV 


==Structural Information==
*Known structures with covalent ligands: 
:[https://www.rcsb.org/structure/1FH0 1FH0] 

*Protein structure
[[File:485.PNG|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04142 Lysosome] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04210 Apoptosis] 

==Experimental Evidence==
:Crystallography

==Reference==
#Somoza J R, Zhan H, Bowman K K, et al. '''Crystal structure of human cathepsin V[J].''' Biochemistry, 2000, 39(41): 12543-12551. [https://www.ncbi.nlm.nih.gov/pubmed/?term=11027133 11027133] 

[[Category:Targets|Targets]]
[[Category:Peptidase C1 family|Peptidase C1 family]]
[[Category:Lysosome|Lysosome]]
[[Category:Apoptosis|Apoptosis]]

@@ Line 44: / Line 44: @@
 #Somoza J R, Zhan H, Bowman K K, et al. '''Crystal structure of human cathepsin V[J].''' Biochemistry, 2000, 39(41): 12543-12551. [https://www.ncbi.nlm.nih.gov/pubmed/?term=11027133 11027133]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Protease|Protease]]
+[[Category:Protease]]
-[[Category:Peptidase C1 family|Peptidase C1 family]]
+[[Category:Peptidase C1 family]]
-[[Category:Lysosome|Lysosome]]
+[[Category:Lysosome]]
-[[Category:Apoptosis|Apoptosis]]
+[[Category:Apoptosis]]

@@ Line 31: / Line 31: @@
 :[https://www.rcsb.org/structure/1FH0 1FH0]<br/>
-*Protein structure
+*Protein structure:
 [[File:485.png|center|800px]]

@@ Line 10: / Line 10: @@
 ===Cys Function & Property===
-Cys138 is one of the active sites of Cathepsin L2, which is very close to His277 and Asn301 in space. These three residues formed a typical catalytic triad motif.<br/>
+:Cys138 is one of the active sites of Cathepsin L2, which is very close to His277 and Asn301 in space. These three residues formed a typical catalytic triad motif.<br/>
 * Hydrophobic property:

@@ Line 2: / Line 2: @@
    | __TOC__
    |}
-{{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys138|[http://pfam.xfam.org/family/PF00112 Peptidase C1 family]|[[:Category:Cathepsin L2|Ligand list]]}}
+{{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys138|[http://pfam.xfam.org/family/PF00112 Peptidase C1 family]|[[:Category:Cathepsin L2|Ligand list]]|Protease}}
 ==Summary==
@@ Line 46: / Line 46: @@
 [[Category:Targets|Targets]]
 [[Category:Homo sapiens|Homo sapiens]]
 [[Category:Peptidase C1 family|Peptidase C1 family]]
 [[Category:Lysosome|Lysosome]]
 [[Category:Apoptosis|Apoptosis]]

@@ Line 32: / Line 32: @@
 *Protein structure
-[[File:485.PNG|center|800px]]
+[[File:485.png|center|800px]]
 ==Related Pathway==

@@ Line 6: / Line 6: @@
 ===Protein Function ===
 Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. (From Wikipedia)<br /> Cathepsin V is a lysosomal cysteine protease that is expressed in the thymus, testis and corneal epithelium. Its sequence shows that it is a member of the papain family of cysteine proteases, and several pieces of evidence demonstrate an especially close link between this enzyme and cathepsin L. The sequences of these two enzymes are quite similar, with a sequence identity of 77% for the proenzyme and 80% for the mature enzyme. In addition, the position and chromosomal organization of the genes coding for these two cathepsins are similar. The cathepsin V gene has been mapped to a region near the cathepsin L locus, and both genes consist of eight exons that code for analogous regions of the cathepsins V and L structures. Cathepsin V may be involved in T-cell selection in humans. (PMID: 11027133)
 ===Cys Function & Property===

Cathepsin L2 - Revision history

Wu3857: /* Reference */

Wu3857 at 20:36, 29 July 2019

Wu3857: /* Reference */

Wu3857: /* Structural Information */

Wu3857 at 17:49, 28 July 2019

Wu3857: /* Structural Information */

Wu3857: /* Protein Function */

Wu3857: /* Cys Function & Property */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys13 8|[http://pfam.xfam.org/family/PF00112..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CTSV|[https://www.uniprot.org/uniprot/O60911 O60911]|Homo sapiens|Cys13 8|[http://pfam.xfam.org/family/PF00112..."