Wu3857: /* Reference */

2019-08-19T21:49:23Z

‎Reference

Wu3857: /* Reference */

2019-08-19T21:48:06Z

‎Reference

Wu3857: /* Structural Information */

2019-07-31T18:31:22Z

‎Structural Information

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CSPB, CTSB|[https://www.uniprot.org/uniprot/P07858 P07858]|Homo sapiens|Cys108|[http://pfam.xfam.org/family/PF0..."

2019-07-31T18:30:25Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CSPB, CTSB|[https://www.uniprot.org/uniprot/P07858 P07858]|Homo sapiens|Cys108|[http://pfam.xfam.org/family/PF0..."

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{{#invoke:InfoboxforTarget|run|CSPB, CTSB|[https://www.uniprot.org/uniprot/P07858 P07858]|Homo sapiens|Cys108|[http://pfam.xfam.org/family/PF00112 Peptidase C1 family]|[[:Category:Cathepsin B (Homo sapiens)|Ligand list]]|Protease}}
==Summary==

===Protein Function ===
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. (From Uniprot) 
Cathepsin B (CatB) is an enzymatic protein belonging to the peptidase (or protease) families. In humans, it is coded by the CTSB gene. The protein encoded by this gene is a lysosomal cysteine protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. At least five transcript variants encoding the same protein have been found for this gene. (From Wikipedia)
 

===Cys Function & Property===
Cys108 is one of the active sites of Cathepsin B, which is very close to His278 and Asn298 in space. These three residues formed a typical catalytic triad motif. 

* Hydrophobic property:
:[[File:509-hydro.png||600px]]
* SASA：
:Cys108: 11.819 A^2 

==Protein Sequence==

MWQLWASLCC LLVLANARSR PSFHPLSDEL VNYVNKRNTT WQAGHNFYNV 
DMSYLKRLCG TFLGGPKPPQ RVMFTEDLKL PASFDAREQW PQCPTIKEIR 
DQGSCGS'''C'''WA FGAVEAISDR ICIHTNAHVS VEVSAEDLLT CCGSMCGDGC 
NGGYPAEAWN FWTRKGLVSG GLYESHVGCR PYSIPPCEHH VNGSRPPCTG 
EGDTPKCSKI CEPGYSPTYK QDKHYGYNSY SVSNSEKDIM AEIYKNGPVE 
GAFSVYSDFL LYKSGVYQHV TGEMMGGHAI RILGWGVENG TPYWLVANSW 
NTDWGDNGFF KILRGQDHCG IESEVVAGIP RTDQYWEKI 


==Structural Information==
*Known structure with covalent ligand: 
:[https://www.rcsb.org/structure/1CSB 1CSB], [https://www.rcsb.org/structure/2IPP 2IPP], [https://www.rcsb.org/structure/1GMY 1GMY] 

*Protein structure:
::[[File:509.png||800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04140 Autophagy - animal] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04142 Lysosome] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04210 Apoptosis] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04612 Antigen processing and presentation] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04621 NOD-like receptor signaling pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04924 Renin secretion] 

==Experimental Evidence==
:Crystallography, MALDI-TOF-MS, Homologous Analysis of Sequence, Enzymatic Assay, Isotope Labeling

==Reference==
# Turk D, Podobnik M, Popovic T, et al. '''Crystal structure of cathepsin B inhibited with CA030 at 2.0-. ANG. resolution: A basis for the design of specific epoxysuccinyl inhibitors[J].''' Biochemistry, 1995, 34(14): 4791-4797. [https://www.ncbi.nlm.nih.gov/pubmed/?term=7718586 7718586] 
# Respondek T, Garner R N, Herroon M K, et al. '''Light activation of a cysteine protease inhibitor: caging of a peptidomimetic nitrile with RuII(bpy)2[J].''' Journal of the American Chemical Society, 2011, 133(43): 17164-17167. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21973207 21973207] 
# Méthot N, Rubin J, Guay D, et al. '''Inhibition of the activation of multiple serine proteases with a cathepsin C inhibitor requires sustained exposure to prevent pro-enzyme processing[J].''' Journal of Biological Chemistry, 2007, 282(29): 20836-20846. [https://www.ncbi.nlm.nih.gov/pubmed/?term=17535802 17535802] 
# Dana D, Davalos A R, De S, et al. '''Development of cell-active non-peptidyl inhibitors of cysteine cathepsins[J].''' Bioorganic & medicinal chemistry, 2013, 21(11): 2975-2987. [https://www.ncbi.nlm.nih.gov/pubmed/?term=23623677 23623677] 
# Greenspan P D, Clark K L, Tommasi R A, et al. '''Identification of dipeptidyl nitriles as potent and selective inhibitors of cathepsin B through structure-based drug design[J].''' Journal of medicinal chemistry, 2001, 44(26): 4524-4534. [https://www.ncbi.nlm.nih.gov/pubmed/?term=11741472 11741472] 
# Wisastra R, Ghizzoni M, Maarsingh H, et al. '''Isothiazolones; thiol-reactive inhibitors of cysteine protease cathepsin B and histone acetyltransferase PCAF[J].''' Organic & biomolecular chemistry, 2011, 9(6): 1817-1822. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21267493 21267493] 
# Falgueyret J P, Black W C, Cromlish W, et al. '''An activity-based probe for the determination of cysteine cathepsin protease activities in whole cells[J].''' Analytical biochemistry, 2004, 335(2): 218-227. [https://www.ncbi.nlm.nih.gov/pubmed/?term=15556560 15556560] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Protease|Protease]]
[[Category:Peptidase C1 family|Peptidase C1 family]]
[[Category:Autophagy - animal|Autophagy - animal]]
[[Category:Lysosome|Lysosome]]
[[Category:Apoptosis|Apoptosis]]
[[Category:Antigen processing and presentation|Antigen processing and presentation]]
[[Category: NOD-like receptor signaling pathway| NOD-like receptor signaling pathway]]
[[Category:Renin secretion|Renin secretion]]

@@ Line 56: / Line 56: @@
 # Falgueyret J P, Black W C, Cromlish W, et al. '''An activity-based probe for the determination of cysteine cathepsin protease activities in whole cells[J].''' Analytical biochemistry, 2004, 335(2): 218-227. [https://www.ncbi.nlm.nih.gov/pubmed/?term=15556560 15556560]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
 [[Category:Homo sapiens]]
 [[Category:Protease]]

@@ Line 57: / Line 57: @@
 [[Category:Targets|Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Protease|Protease]]
+[[Category:Protease]]
-[[Category:Peptidase C1 family|Peptidase C1 family]]
+[[Category:Peptidase C1 family]]
-[[Category:Autophagy - animal|Autophagy - animal]]
+[[Category:Autophagy - animal]]
-[[Category:Lysosome|Lysosome]]
+[[Category:Lysosome]]
-[[Category:Apoptosis|Apoptosis]]
+[[Category:Apoptosis]]
-[[Category:Antigen processing and presentation|Antigen processing and presentation]]
+[[Category:Antigen processing and presentation]]
-[[Category: NOD-like receptor signaling pathway| NOD-like receptor signaling pathway]]
+[[Category: NOD-like receptor signaling pathway]]
-[[Category:Renin secretion|Renin secretion]]
+[[Category:Renin secretion]]

@@ Line 34: / Line 34: @@
 *Protein structure:
-::[[File:509.png||800px]]
+::[[File:509.png|center|800px]]
 ==Related Pathway==

Cathepsin B (Homo sapiens) - Revision history

Wu3857: /* Reference */

Wu3857: /* Reference */

Wu3857: /* Structural Information */

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|CSPB, CTSB|[https://www.uniprot.org/uniprot/P07858 P07858]|Homo sapiens|Cys108|[http://pfam.xfam.org/family/PF0..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|CSPB, CTSB|[https://www.uniprot.org/uniprot/P07858 P07858]|Homo sapiens|Cys108|[http://pfam.xfam.org/family/PF0..."