Arachidonate 5-lipoxygenase - Revision history

Wu3857: /* Reference */

2019-08-19T21:37:45Z

‎Reference

Wu3857: /* Protein Function */

2019-08-05T02:24:29Z

‎Protein Function

Wu3857: /* Reference */

2019-08-05T02:22:44Z

‎Reference

Wu3857: /* Protein Function */

2019-08-05T02:19:24Z

‎Protein Function

Wu3857 at 02:19, 5 August 2019

2019-08-05T02:19:08Z

Wu3857 at 02:16, 5 August 2019

2019-08-05T02:16:44Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|5-LO, ALOX5|[https://www.uniprot.org/uniprot/P09917 P09917]|Homo sapiens|Cys417|[http://pfam.xfam.org/family/PF..."

2019-08-05T02:16:30Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|5-LO, ALOX5|[https://www.uniprot.org/uniprot/P09917 P09917]|Homo sapiens|Cys417|[http://pfam.xfam.org/family/PF..."

New page

{| align="left"
| __TOC__
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{{#invoke:InfoboxforTarget|run|5-LO, ALOX5|[https://www.uniprot.org/uniprot/P09917 P09917]|Homo sapiens|Cys417|[http://pfam.xfam.org/family/PF00305 Lipoxygenase family]|[[:Category:Arachidonate 5-lipoxygenase|Ligand list]]|Metabolic enzyme}}

==Summary==

===Protein Function ===
Lipoxygenases are a family of iron-containing enzymes that catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4-pentadiene structure. It catalyses the following reaction:
'''fatty acid + O2 = fatty acid hydroperoxide'''
These enzymes are most common in plants where they may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leu*[https://www.genome.jp/kegg-bin/show_pathway?kotrienes and nonclassic eicosanoids). (From Wikipedia)
5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of leu*[https://www.genome.jp/kegg-bin/show_pathway?kotrienes (LTs) which are mediators involved in the development of atherosclerosis, inflammatory diseases and cancer. 5-LO metabolizes arachidonic acid to leu*[https://www.genome.jp/kegg-bin/show_pathway?kotriene A4 in a two step reaction by incorporation of molecular oxygen. (PMID: 22137889)
 

===Cys Function & Property===
Cys417 is close to the active site and the Fe cation in space. 

* Hydrophobic property:
:[[File:529-hydro.png||600px]]
* SASA：
:Cys417: 26.389 A^2

==Protein Sequence==

MPSYTVTVAT GSQWFAGTDD YIYLSLVGSA GCSEKHLLDK PFYNDFERGA 
VDSYDVTVDE ELGEIQLVRI EKRKYWLNDD WYLKYITLKT PHGDYIEFPC 
YRWITGDVEV VLRDGRAKLA RDDQIHILKQ HRRKELETRQ KQYRWMEWNP 
GFPLSIDAKC HKDLPRDIQF DSEKGVDFVL NYSKAMENLF INRFMHMFQS 
SWNDFADFEK IFVKISNTIS ERVMNHWQED LMFGYQFLNG CNPVLIRRCT 
ELPEKLPVTT EMVECSLERQ LSLEQEVQQG NIFIVDFELL DGIDANKTDP 
CTLQFLAAPI CLLYKNLANK IVPIAIQLNQ IPGDENPIFL PSDAKYDWLL 
AKIWVRSSDF HVHQTITHLL RTHLVSEVFG IAMYRQLPAV HPIFKLLVAH 
VRFTIAINTK AREQLI'''C'''ECG LFDKANATGG GGHVQMVQRA MKDLTYASLC 
FPEAIKARGM ESKEDIPYYF YRDDGLLVWE AIRTFTAEVV DIYYEGDQVV 
EEDPELQDFV NDVYVYGMRG RKSSGFPKSV KSREQLSEYL TVVIFTASAQ 
HAAVNFGQYD WCSWIPNAPP TMRAPPPTAK GVVTIEQIVD TLPDRGRSCW 
HLGAVWALSQ FQENELFLGM YPEEHFIEKP VKEAMARFRK NLEAIVSVIA 
ERNKKKQLPY YYLSPDRIPN SVAI 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:529.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00590 Arachidonic acid metabolism] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04664 Fc epsilon RI signaling pathway] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04726 Serotonergic synapse] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko04913 Ovarian steroidogenesis] 
*[https://www.genome.jp/kegg-bin/show_pathway?ko05145 Toxoplasmosis] 

==Experimental Evidence==
:Cys-directed Mutation, MALDI-MS

==Reference==
# Hörnig M, Mar*[https://www.genome.jp/kegg-bin/show_pathway?koutsa S, Häfner A K, et al. '''Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416[J].''' Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2012, 1821(2): 279-286. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22137889 22137889] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Metabolic enzyme|Metabolic enzyme]]
[[Category:Lipoxygenase family|Lipoxygenase family]]
[[Category:Arachidonic acid metabolism|Arachidonic acid metabolism]]
[[Category:Metabolic pathways|Metabolic pathways]]
[[Category:Serotonergic synapse|Serotonergic synapse]]
[[Category:Ovarian steroidogenesis|Ovarian steroidogenesis]]
[[Category:Toxoplasmosis|Toxoplasmosis]]

@@ Line 59: / Line 59: @@
 # Hörnig M, Markoutsa S, Häfner A K, et al. '''Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416[J].''' Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2012, 1821(2): 279-286. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22137889 22137889]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Metabolic enzyme|Metabolic enzyme]]
+[[Category:Metabolic enzyme]]
-[[Category:Lipoxygenase family|Lipoxygenase family]]
+[[Category:Lipoxygenase family]]
-[[Category:Arachidonic acid metabolism|Arachidonic acid metabolism]]
+[[Category:Arachidonic acid metabolism]]
-[[Category:Metabolic pathways|Metabolic pathways]]
+[[Category:Metabolic pathways]]
-[[Category:Serotonergic synapse|Serotonergic synapse]]
+[[Category:Serotonergic synapse]]
-[[Category:Ovarian steroidogenesis|Ovarian steroidogenesis]]
+[[Category:Ovarian steroidogenesis]]
-[[Category:Toxoplasmosis|Toxoplasmosis]]
+[[Category:Toxoplasmosis]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
-Lipoxygenases (EC 1.13.11.-) are a family of iron-containing enzymes that catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4-pentadiene structure. It catalyses the following reaction: <br/>
+Lipoxygenases are a family of iron-containing enzymes that catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4-pentadiene structure. It catalyses the following reaction: <br/>
 '''fatty acid + O2 = fatty acid hydroperoxide'''<br/>
 These enzymes are most common in plants where they may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leukotrienes and nonclassic eicosanoids). (From Wikipedia)<br/>

@@ Line 57: / Line 57: @@
 ==Reference==
-# Hörnig M, Mar*[https://www.genome.jp/kegg-bin/show_pathway?koutsa S, Häfner A K, et al. '''Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416[J].''' Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2012, 1821(2): 279-286. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22137889 22137889]<br/>
+# Hörnig M, Markoutsa S, Häfner A K, et al. '''Inhibition of 5-lipoxygenase by U73122 is due to covalent binding to cysteine 416[J].''' Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2012, 1821(2): 279-286. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22137889 22137889]<br/>
 [[Category:Targets|Targets]]

@@ Line 7: / Line 7: @@
 ===Protein Function ===
-Lipoxygenases (EC 1.13.11.-) are a family of iron-containing enzymes that catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4-pentadiene structure. It catalyses the following reaction:
+Lipoxygenases (EC 1.13.11.-) are a family of iron-containing enzymes that catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4-pentadiene structure. It catalyses the following reaction: <br/>
-'''fatty acid + O2 = fatty acid hydroperoxide'''
+'''fatty acid + O2 = fatty acid hydroperoxide'''<br/>
-These enzymes are most common in plants where they may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leukotrienes and nonclassic eicosanoids). (From Wikipedia)
+These enzymes are most common in plants where they may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leukotrienes and nonclassic eicosanoids). (From Wikipedia)<br/>
 -Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of leukotrienes (LTs) which are mediators involved in the development of atherosclerosis, inflammatory diseases and cancer. 5-LO metabolizes arachidonic acid to leukotriene A4 in a two step reaction by incorporation of molecular oxygen. (PMID: 22137889)<br/>

@@ Line 10: / Line 10: @@
 '''fatty acid + O2 = fatty acid hydroperoxide'''
 These enzymes are most common in plants where they may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. In mammals a number of lipoxygenases isozymes are involved in the metabolism of eicosanoids (such as prostaglandins, leu*[https://www.genome.jp/kegg-bin/show_pathway?kotrienes and nonclassic eicosanoids).  (From Wikipedia)
--Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of leu*[https://www.genome.jp/kegg-bin/show_pathway?kotrienes (LTs) which are mediators involved in the development of atherosclerosis, inflammatory diseases and cancer.  5-LO metabolizes arachidonic acid to leu*[https://www.genome.jp/kegg-bin/show_pathway?kotriene A4 in a two step reaction by incorporation of molecular oxygen. (PMID: 22137889)
+-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of leu*[https://www.genome.jp/kegg-bin/show_pathway?kotrienes (LTs) which are mediators involved in the development of atherosclerosis, inflammatory diseases and cancer.  5-LO metabolizes arachidonic acid to leu*[https://www.genome.jp/kegg-bin/show_pathway?kotriene A4 in a two step reaction by incorporation of molecular oxygen. (PMID: 22137889)<br/>
 ===Cys Function & Property===

Arachidonate 5-lipoxygenase - Revision history

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857: /* Reference */

Wu3857: /* Protein Function */

Wu3857 at 02:19, 5 August 2019

Wu3857 at 02:16, 5 August 2019

Wu3857: Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|5-LO, ALOX5|[https://www.uniprot.org/uniprot/P09917 P09917]|Homo sapiens|Cys417|[http://pfam.xfam.org/family/PF..."

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|5-LO, ALOX5|[https://www.uniprot.org/uniprot/P09917 P09917]|Homo sapiens|Cys417|[http://pfam.xfam.org/family/PF..."