Actin, alpha skeletal muscle (Homo sapiens) - Revision history

Wu3857: /* Cys Function & Property */

2020-01-22T05:04:59Z

‎Cys Function & Property

Wu3857: /* Protein Function */

2020-01-22T05:04:49Z

‎Protein Function

Wu3857: /* Reference */

2019-08-19T21:36:47Z

‎Reference

Wu3857: /* Structural Information */

2019-08-09T21:55:57Z

‎Structural Information

Wu3857 at 21:55, 9 August 2019

2019-08-09T21:55:47Z

Wu3857: Created page with "{| align="left" | TOC |} {{#invoke:InfoboxforTarget|run|ACTA1|[https://www.uniprot.org/uniprot/P68133 P68133]|Homo sapiens|Cys259, Cys376|[http://pfam.xfam.org/family/..."

2019-08-09T21:55:29Z

Created page with "{| align="left" | __TOC__ |} {{#invoke:InfoboxforTarget|run|ACTA1|[https://www.uniprot.org/uniprot/P68133 P68133]|Homo sapiens|Cys259, Cys376|[http://pfam.xfam.org/family/..."

New page

{| align="left"
| __TOC__
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{{#invoke:InfoboxforTarget|run|ACTA1|[https://www.uniprot.org/uniprot/P68133 P68133]|Homo sapiens|Cys259, Cys376|[http://pfam.xfam.org/family/PF00022 Actin family]|[[:Category:Actin, alpha skeletal muscle (Homo sapiens)|Ligand list]]|Structural protein}}

==Summary==

===Protein Function ===
Actin, alpha skeletal muscle expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. 
Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. Such conditions result in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene. SRF may bring a number of other proteins to the promoter of skeletal actin, such as andogen receptor, and thereby contribute to induction of skeletal actin gene expression by androgenic (often termed "anabolic") steroids. (From Wikipedia) 
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. (From Uniprot)
 

===Cys Function & Property===
Cys259 is close to the ATP binding site of ACTA1. 
Cys374, the most reactive and surface-exposed thiol group in 5 cysteine reisudes in rat Actin, is located at the C-terminal, and the covalent modification maybe influence the interaction with other protein. 

* Hydrophobic property:
:[[File:541-hydro.png||600px]]
* SASA：
:Cys259: Unknown
:Cys376: Unknown

==Protein Sequence==

MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG 
QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP 
EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT 
TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY 
SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI 
TIGNERFR'''C'''P ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV 
MSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS 
LSTFQQMWIT KQEYDEAGPS IVHRK'''C'''F 


==Structural Information==
*Known structures with covalent ligands: 
:Unknown 

*Protein structure:
[[File:541.png|center|800px]]
<div align="center">Homology structure from Rat alpha-Actin</div> 

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04260 Cardiac muscle contraction] 

==Experimental Evidence==
:Mass Spectrometry, Pepsin Digest

==Reference==
# Sörensen P M, Iacob R E, Fritzsche M, et al. '''The natural product cucurbitacin E inhibits depolymerization of actin filaments[J].''' ACS chemical biology, 2012, 7(9): 1502-1508. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22724897 22724897] 
# Dalle-Donne I, Carini M, Vistoli G, et al. '''Actin Cys374 as a nucleophilic target of α, β-unsaturated aldehydes[J].''' Free Radical Biology and Medicine, 2007, 42(5): 583-598. [https://www.ncbi.nlm.nih.gov/pubmed/?term=17291982 17291982] 

[[Category:Targets|Targets]]
[[Category:Homo sapiens|Homo sapiens]]
[[Category:Structural protein|Structural protein]]
[[Category:Actin family|Actin family]]
[[Category:Cardiac muscle contraction|Cardiac muscle contraction]]

@@ Line 15: / Line 15: @@
 ===Cys Function & Property===
 Cys259 is close to the ATP binding site of ACTA1.<br/>
 Cys374, the most reactive and surface-exposed thiol group in 5 cysteine reisudes in rat Actin, is located at the C-terminal, and the covalent modification maybe influence the interaction with other protein.<br/>

@@ Line 8: / Line 8: @@
 ===Protein Function ===
 Actin, alpha skeletal muscle expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. <br/>
 Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. Such conditions result in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene. SRF may bring a number of other proteins to the promoter of skeletal actin, such as andogen receptor, and thereby contribute to induction of skeletal actin gene expression by androgenic (often termed "anabolic") steroids. (From Wikipedia) <br/>
 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. (From Uniprot)<br/>

@@ Line 51: / Line 51: @@
 # Dalle-Donne I, Carini M, Vistoli G, et al. '''Actin Cys374 as a nucleophilic target of α, β-unsaturated aldehydes[J].''' Free Radical Biology and Medicine, 2007, 42(5): 583-598. [https://www.ncbi.nlm.nih.gov/pubmed/?term=17291982 17291982]<br/>
-[[Category:Targets|Targets]]
+[[Category:Targets]]
-[[Category:Homo sapiens|Homo sapiens]]
+[[Category:Homo sapiens]]
-[[Category:Structural protein|Structural protein]]
+[[Category:Structural protein]]
-[[Category:Actin family|Actin family]]
+[[Category:Actin family]]
-[[Category:Cardiac muscle contraction|Cardiac muscle contraction]]
+[[Category:Cardiac muscle contraction]]

@@ Line 40: / Line 40: @@
 [[File:542.png|center|800px]]
 <div align="center">Homology structure from Rat alpha-Actin</div><br/>
 ==Related Pathway==

@@ Line 9: / Line 9: @@
 Actin, alpha skeletal muscle expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. <br/>
 Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. Such conditions result in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene. SRF may bring a number of other proteins to the promoter of skeletal actin, such as andogen receptor, and thereby contribute to induction of skeletal actin gene expression by androgenic (often termed "anabolic") steroids. (From Wikipedia) <br/>
-Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. (From Uniprot)
+Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. (From Uniprot)<br/>
 ===Cys Function & Property===
@@ Line 17: / Line 16: @@
 * Hydrophobic property:
-:[[File:541-hydro.png||600px]]
+:[[File:542-hydro.png||600px]]
 * SASA：
 :Cys259: Unknown
@@ Line 39: / Line 38: @@
 *Protein structure:
-[[File:541.png|center|800px]]
+[[File:542.png|center|800px]]
 <div align="center">Homology structure from Rat alpha-Actin</div><br/>